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RND3 Antikörper (Center)

RND3 Reaktivität: Human, Maus WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN2496515
  • Target Alle RND3 Antikörper anzeigen
    RND3 (rho Family GTPase 3 (RND3))
    Bindungsspezifität
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Center
    Reaktivität
    • 30
    • 7
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    Human, Maus
    Wirt
    • 16
    • 14
    Kaninchen
    Klonalität
    • 16
    • 14
    Polyklonal
    Konjugat
    • 19
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser RND3 Antikörper ist unkonjugiert
    Applikation
    • 16
    • 13
    • 7
    • 6
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
    Homologie
    Human Mouse
    Immunogen
    This ARHE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 142~171 amino acids from the Center region of human AEHE.
    Isotyp
    Ig Fraction
    Top Product
    Discover our top product RND3 Primärantikörper
  • Applikationshinweise
    Optimal working dilution should be determined by the investigator.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Validierung #100103 (Western Blotting)
    'Independent Validation' Siegel
    by
    Instituto de Parasitología y Biomedicina López-Neyra
    No.
    #100103
    Datum
    23.12.2016
    Antigen
    RND3
    Chargennummer
    SH080408I
    Validierte Anwendung
    Western Blotting
    Positivkontrolle
    primary mouse microglia
    Negativkontrolle
    siRNA knock-down of RND3 in primary mouse microglia
    Bewertung
    Passed. ABIN2496515 specifically recognizes Rnd3 in primary mouse microglia lysates.
    'Independent Validation' Siegel
    Validierungsbilder
    Protokoll
    Primärantikörper
    ABIN2496515
    Sekundärantikörper
    anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
    Full Protocol
    • Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
    • Transfect cells with cells with 0.05nmol mouse RND3 Silencer Select siRNA (ThermoFisher Scientific, ASO10960, ASO109AE and ASO109FC, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer´s instructions.
    • Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma)).
    • Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
    • Denature 5µg total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing freshly cast 10% SDS-PAGE for 1h at 140V.
    • Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
    • Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
    • Incubation with primary mouse anti-RND3 antibody (antibodies-online, ABIN2496515, lot SH080408I) diluted 1:500 in TBST containing 5% milk for 1h at RT.
    • Wash membrane 5x 5min with TBST.
    • Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
    • Wash membrane 5x 5min with TBST and 3x 5min with TBS.
    • Reveal protein bands using ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232, lot 9547481) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
    • Strip membranes using RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
    • Wash and reveal the protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
    Anmerkungen
    The RND3 antibody ABIN2496515 reveals a protein of the expected molecular weight of murine Rnd3 in lysates of primary mouse microglia. The protein band’s intensity decreases upon siRNA knockdown.
  • Format
    Liquid
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
    Haltbarkeit
    6 months
  • Target
    RND3 (rho Family GTPase 3 (RND3))
    Andere Bezeichnung
    RND3 (RND3 Produkte)
    Synonyme
    ARHE antikoerper, Rho8 antikoerper, RhoE antikoerper, memB antikoerper, MGC83857 antikoerper, 2610017M01Rik antikoerper, AI661404 antikoerper, Arhe antikoerper, Rhoe antikoerper, RHOE antikoerper, arhe antikoerper, id:ibd2712 antikoerper, id:ibd2919 antikoerper, rho8 antikoerper, rnd3 antikoerper, wu:fc39h07 antikoerper, zgc:73080 antikoerper, wu:fd06e12 antikoerper, zgc:92324 antikoerper, Rho family GTPase 3 antikoerper, Rho family GTPase 3 L homeolog antikoerper, Rho family GTPase 3a antikoerper, Rho family GTPase 3b antikoerper, RND3 antikoerper, rnd3.L antikoerper, Rnd3 antikoerper, rnd3a antikoerper, rnd3b antikoerper
    Molekulargewicht
    27368 Da
    Gen-ID
    390
    NCBI Accession
    NP_001241667
    UniProt
    P61587
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