Exoglucanase Isoenzyme 1 (EXO1) Antikörper

Antigen: Exoglucanase Isoenzyme 1 (EXO1)

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Barley (Hordeum vulgare)

Applikation: Western Blot (WB), ELISA

Produktnummer: ABIN249341

Produktbeschreibung

Immunogen: synthetic peptide conjugated to KLH

Format: Lyophilized

Beschreibung: Family 3 beta-d-glucan glucohydrolases are widely distributed in higher plants.The enzymes catalyse the hydrolytic removal of beta-d-glucosyl residues fromnon-reducing termini of a range of beta-d-glucans and beta-d-oligoglucosides.Their broad specificity can be rationalized from X-ray crystallographic dataobtained from a barley beta-d-glucan glucohydrolase in complex withnon-hydrolysable S-glycoside substrate analogues, and from molecular modellingof enzyme-substrate complexes. The glucosyl residue occupying binding subsite-1 is tightly locked into a fixed position through extensive hydrogen bonding withsix amino acid residues near the bottom of an active site pocket. In contrast, theglucosyl residue at subsite +1 is located between two tryptophan residues at theentrance of the pocket, where it is less tightly constrained. The relative flexibility ofbinding at subsite +1, coupled with the projection of the remainder of boundsubstrate away from the enzyme’s surface, means that the overall active site canaccommodate a range of substrates with variable spatial dispositions of adjacentbeta-d-glucosyl residues. The broad specificity for glycosidic linkage type wouldenable the enzyme to perform diverse functions during plant development.

Spezifität: Predicted reactivity: Oryza sativa, Zea mays.

Anwendungen

Applikationshinweise: Recommended Dilution 1: 5 000 with standard ECL (WB) , 1: 10 000 (ELISA).

Reinheit: Serum

Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior toopening them to avoid any losses that might occur from lyophilized materialadhering to the cap or sides of the tubes.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Hrmova, De Gori, Smith et al.: "Structural basis for broad substrate specificity in higher plant beta-D-glucan glucohydrolases." in: The Plant cell, Vol. 14, Issue 5, pp. 1033-52, 2002 (PubMed).