XTH-Xet Antikörper

Produktnummer ABIN249338

Produktdetails

Target: XTH-Xet

Reaktivität: Gerste, Oryza sativa

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: ELISA, Western Blotting (WB)

Kreuzreaktivität (Details): Not reactive in: Zea mays

Homologie: monocots

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 31.5 / 33 kDa

Aufreinigung: serum

Immunogen: two synthetic peptides from highly conserved region of Horderum vulgare XTH-Xet

Anwendungsinformationen

Applikationshinweise: 1: 500 with standard ECL (WB) , 1: 5000 (ELISA)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water.

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen

Hrmova, Farkas, Lahnstein, Fincher: "A Barley xyloglucan xyloglucosyl transferase covalently links xyloglucan, cellulosic substrates, and (1,3;1,4)-beta-D-glucans." in: The Journal of biological chemistry, Vol. 282, Issue 17, pp. 12951-62, (2007) (PubMed).

Antigendetails

Target: XTH-Xet

Hintergrund: Molecular interactions between wall polysaccharides, which include cellulose and a range of non-cellulosic polysaccharides such as xyloglucans and (1,3,1,4)-β-d-glucans, are fundamental to cell wall properties. These interactions have been assumed to be non-covalent in nature in most cases. A highly purified barley xyloglucan xyloglucosyl transferase HvXET5 (EC 2.4.1.207), a member of the GH16 group of glycoside hydrolases, catalyses the in vitro formation of covalent linkages between xyloglucans and cellulosic substrates, and between xyloglucans and (1,3,1,4)-β-d-glucans. It is possible that XETs could link different polysaccharides in vivo, and hence influence cell wall strength, flexibility and porosity.

Molekulargewicht: expected: 31.5 kDa, apparent: 33 kDa