TRPC5 Antikörper (AA 827-845) (FITC)

Produktnummer ABIN2485379

Produktdetails anti-TRPC5 Antikörper (FITC)

Target: TRPC5 (Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5))

Bindungsspezifität: AA 827-845

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TRPC5 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Antibody Array (AA)

Spezifität: Detects ~110 kDa.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: Synthetic peptide amino acids 827-845 of human TrpC5 (also known as short transient receptor potential channel 5, and Htrp5)

Klon: N67-15 (Formerly S67-15)

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise:

• WB (1:1000)
• IHC (1:1000)
• ICC/IF (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2485379 was sufficient for detection of TrpC5 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Independent Validation (IF)

Validierung #103875 (Immunofluorescence)

by: Experimentelle Anästhesiologie, Universitätsklinikum Erlangen

No.: #103875

Datum: 10.04.2019

Antigen: TRPC5

Chargennummer: 1007

Validierte Anwendung: Immunofluorescence

Positivkontrolle:

C57Bl6/J Mouse WT Trigeminal Ganglion (TG)

Negativkontrolle:

Mouse TRPC5 knock-out Trigeminal Ganglion

Bewertung:

ABIN2485379 specifically stains TRPC5 positive neurons in fixed murine trigeminal ganglia.

Validierungsbilder

A. Representative image of ophthalmic region of trigeminal ganglion of C57Bl6/J mouse stained with ABIN2485379 diluted 1:500. B. Representative image of Z-Stack of ophthalmic region of trigeminal ganglion of TRPC5 ko stained with ABIN2485379 diluted 1:500.

Protokoll

Primärantikörper: ABIN2485379

Full Protocol:

• Surgical plane of anesthesia with Ketamine and Xylazine.
• Perfuse mouse transcardially with
• • 1ml/g BW ice cold PBS
  • 1ml/g BW 4% PFA.
• Postfix skull base in 4% PFA ON at 4°C.
• Harvest TGs in PBS.
• Wash TGs 4x with PBS for 30min at RT.
• Incubate TGs with rotator in blocking solution (PBS containing 1% BSA, 0.5% Triton X-100) ON at 4°C.
• Incubate TGs with rotator with primary FITC-conjugated mouse anti-TRPC5 antibody (antibodies-online, ABIN2485379, lot 1007) diluted 1:500 in blocking solution ON at 4°C.
• Wash TGs 4x with PBS for 30min at RT.
• Mount TGs with 1% low melting agarose.
• Whole Organ 2-Photon Imaging with a Zeiss LSM880 NLO Intravital Microscope; Settings: Excitation Wavelenth: 940 nm, Detection: 500-550 nm for wt and TRPC5 ko TGs.

Anmerkungen:

• In wt mice antibody concentrations of 1:100, 1:500, 1:1000 and 1:2000 were tested with best results using a concentration of 1:500.

• ABIN2485379 is suitable for whole organ IHC. TRPC5 positive Neurons show clear cytoplasmic and membranous staining. No neuronal staining visible. Residues of antibody visible in vasculature due to tissue manipulation during washing.

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu TRPC5

Target: TRPC5 (Transient Receptor Potential Cation Channel, Subfamily C, Member 5 (TRPC5))

Andere Bezeichnung: TRPC5 (TRPC5 Produkte)

Synonyme: trpc5 antikoerper, wu:fi73f08 antikoerper, TRPC5 antikoerper, Trpc5 antikoerper, TRP5 antikoerper, CCE2 antikoerper, TRP-5 antikoerper, Trrp5 antikoerper, trp antikoerper, transient receptor potential cation channel, subfamily C, member 5a antikoerper, transient receptor potential cation channel subfamily C member 5 antikoerper, transient receptor potential cation channel, subfamily C, member 5 antikoerper, trpc5a antikoerper, TRPC5 antikoerper, Trpc5 antikoerper

Hintergrund: Transient receptor potential cation channel, subfamily C, member 5, also known as TRPC5, is a subtype of the TRPC family of mammalian transient receptor potential ion channels. Homo-multimeric TRPC5 and hetero-multimeric TRPC5-TRPC1 channels are activated by extracellular reduced thioredoxin (1). This activation probably plays a role in rheumatoid arthritis. It has also been recently found to be involved in the action on anaesthetics such as chloroform, halothane and propofol (2).

Gen-ID: 7224

NCBI Accession: NP_036603

UniProt: Q9UL62