HSPB8 Antikörper (FITC)

Produktnummer ABIN2484237

Produktdetails anti-HSPB8 Antikörper (FITC)

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser HSPB8 Antikörper ist konjugiert mit FITC

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Spezifität: Detects ~22 kDa. Detects endogenous and exogenous HSP22 in monomeric, dimeric and tetrameric forms in WB. Does not cross react with alpha crystallin.

Kreuzreaktivität: Human, Maus, Ratte

Aufreinigung: Protein G Purified

Immunogen: His-tagged human recombinant HSP22

Klon: 3C12-H11

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise:

• WB (1:2000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Kommentare:

1 μg/ml of ABIN2484237 was sufficient for detection of HSP22 in 20 μg of whole rat tissue extract by ECL immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Conjugated antibodies should be stored at 4°C

Details zu HSPB8

Target: HSPB8 (Heat Shock 22kDa Protein 8 (HSPB8))

Andere Bezeichnung: HSP22 (HSPB8 Produkte)

Synonyme: CMT2L antikoerper, DHMN2 antikoerper, E2IG1 antikoerper, H11 antikoerper, HMN2 antikoerper, HMN2A antikoerper, HSP22 antikoerper, MGC64408 antikoerper, hsc70 antikoerper, wu:fb01g06 antikoerper, wu:fi48b06 antikoerper, fc09c11 antikoerper, wu:fc04b04 antikoerper, wu:fc09c11 antikoerper, zgc:64202 antikoerper, AU018630 antikoerper, AW413033 antikoerper, Cryac antikoerper, D5Ucla4 antikoerper, H11K antikoerper, HSP20-like antikoerper, Hsp22 antikoerper, heat shock protein family B (small) member 8 antikoerper, heat shock protein family B (small) member 8 L homeolog antikoerper, heat shock protein 8 antikoerper, heat shock protein b8 antikoerper, HSPB8 antikoerper, hspb8.L antikoerper, hspa8 antikoerper, hspb8 antikoerper, Hspb8 antikoerper

Hintergrund: HSP27s belong to an abundant and ubiquitous family of small heat shock proteins (sHSP). It is an important HSP found in both normal human cells and cancer cells. The basic structure of most sHSPs is a homologous and highly conserved amino acid sequence, with an α-crystallin domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (1, 2). HSP27-oligomers consist of stable dimers formed by as many as 8-40 HSP27 protein monomers (3). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (4). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. Other functions include chaperone activity (as mentioned above), thermo tolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. HSP27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that HSP27 may serve some role in cross-bridge formation between actin and myosin (5). And finally, HSP27 is also thought to be involved in the process of cell differentiation. The up-regulation of HSP27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that HSP27 may play a crucial role in termination of growth (6).

Gen-ID: 26353

NCBI Accession: NP_055180

UniProt: Q9UJY1