LEFTY2 Antikörper

Produktnummer ABIN2470659

Produktdetails anti-LEFTY2 Antikörper

Target: LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LEFTY2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Spezifität: A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.

Aufreinigung: Protein A Column

Immunogen: LEFTY2 monoclonal antibody was raised against recombinant LEFTY2 (Human). Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells using conventional hybridoma technology.

Klon: 7C5G1H6H10

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: LEFTY2 antibody can be used in immunohistochemistry starting at 2.5 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: As with all antibodies avoid freeze/thaw cycles.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store LEFTY2 antibody at 4 °C or -20 °C.

Details zu LEFTY2

Target: LEFTY2 (Left-Right Determination Factor 2 (LEFTY2))

Andere Bezeichnung: LEFTY2 (LEFTY2 Produkte)

Synonyme: EBAF antikoerper, LEFTA antikoerper, LEFTYA antikoerper, TGFB4 antikoerper, AI450052 antikoerper, Ebaf antikoerper, Leftb antikoerper, Stra3 antikoerper, Tgfb4 antikoerper, lefty antikoerper, lefty-1 antikoerper, atv2 antikoerper, cb720 antikoerper, fe38b03 antikoerper, wu:fe38b03 antikoerper, 6030463A22Rik antikoerper, AV214969 antikoerper, Lefta antikoerper, Ebaf2 antikoerper, left-right determination factor 2 antikoerper, left right determination factor 1 antikoerper, lefty2 antikoerper, Left-right determination factor 2 antikoerper, left-right determination factor 2-like antikoerper, LEFTY2 antikoerper, Lefty1 antikoerper, lft2 antikoerper, Lefty2 antikoerper, LOC699676 antikoerper, LOC100232580 antikoerper

Hintergrund: During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-beta family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-beta (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-beta actions are directed by ligand-induced activation of TGF-beta receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-beta superfamily, inhibits TGF-beta signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-beta receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-beta-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-beta superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 kDa) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.

Gen-ID: 7044

UniProt: O00292