ANKK1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ANKK1 antibody was raised against a 15 amino acid synthetic peptide near the internal region of ANKK1.
ANKK1
Reaktivität: Maus, Ratte
WB, IF (p), IF (cc)
Wirt: Kaninchen
Polyclonal
Alexa Fluor 488
Applikationshinweise
ELISA: Antibody detection limit dilution 1:128000. Approximately 85 kDa band observed in human brain (Substantia nigra and amygdala) lysates (calculated MW of 84.6 kDa according to NP_848605.1). Recommended concentration: 0.1-0.3 μg/mL. an additional band of unknown identity was also consistently observed at 35 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
ANKK1
(Ankyrin Repeat and Protein Kinase Domain-Containing Protein 1 (ANKK1))