SPEN antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SPEN antibody was raised against a 15 amino acid synthetic peptide near the internal region of SPEN.
ELISA: Antibody detection limit dilution 1:4,000. Western Blot: Not yet tested - our routinely used Western Blotting protocol does not allow detection of proteins as large as the calculated size of approximately 402 kDa according to NP_055816.2. Therefore we cannot recommend an optimal concentration and the product is investigative grade.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.