Dieser alpha-Mannosidase II Antikörper ist unkonjugiert
Applikation
Western Blotting (WB), ELISA
Aufreinigung
MAN2A1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
MAN2A1 antibody was raised against an 11 amino acid synthetic peptide near the internal region of MAN2A1.
MAN2A1
Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
Biotin
Applikationshinweise
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 150 kDa band observed in human placenta lysates (calculated MW of 131 kDa according to NP_002363.2). In transfected HEK293 transiently expressing MAN2A1 a band of approximately 98 kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 1-3 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
alpha-Mannosidase II (MAN2A1)
(Mannosidase, Alpha, Class 2A, Member 1 (MAN2A1))