Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Aufreinigung
Lipocalin 2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Lipocalin 2 antibody was raised against a 13 amino acid synthetic peptide near the internal region of Lipocalin 2.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 22 kDa band observed in human kidney lysates (calculated MW of 22.6 kDa according to NP_005555.2). Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded human spleen shows staining of cytoplasm in mostly binucleated cells, and of nucleoli in some of the non-dividing cells. Recommended concentration, 2-3 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.