Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Aufreinigung
GULP1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GULP1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of GULP1.
GULP1
Reaktivität: Human, Maus
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro), ICC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 35 kDa band observed in human placenta lysates (calculated MW of 34.5 kDa according to NP_057399.1). Recommended concentration: 0.2-0.6 μg/mL. an additional band of unknown identity was also consistently observed at approximately46 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human kidney shows staining of cytoplasm, stronger in a selection of epithelial cells of distal convoluted tubules. Recommended concentration, 5-10 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.