Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP) (AA 662-672) Antikörper

Antigen: Collagen, Type IV, alpha 3 (Goodpasture Antigen) Binding Protein (COL4A3BP)

Synonyme: COL4A3BP, col4a3bp, zgc:92147, wu:fc37f11, CERT, GPBP, AU016711, 2810404O15Rik, 9230101K08Rik, CERTL, STARD11, FLJ20597, cetp, MGC80009

Epitope: AA 662-672

Klonalität: Polyklonal

Wirt: Ziege

Reaktivität: Maus

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN238598

Produktbeschreibung

Weitere Bezeichnung: COL4A3BP (aa662-672)

Gen-ID: 10087, 68018 (mouse)

Immunogen: DHDSAPLNNRC

Format: Affinity-purified

Beschreibung: All names and symbols: COL4A3BP, collagen, type IV, alpha 3 (Goodpasture antigen) binding protein , CERT, CERTL, FLJ20597, GPBP , STARD11, Goodpasture antigen-binding protein, START domain containing 11, alpha 3 type IV collagen binding protein, ceramide transporter,. Official Symbol: COL4A3BP. RefSeq number(s): NP_005704.1, NP_112729.1

Spezifität: This antibody is expected to recognise both reported isoforms (NP_005704.1 and NP_112729.1) Human, Mouse

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 60kDa band observed in mouse heart lysates (calculated MW of 71.1kDa according to mouse NP_075909.1 and 60.0kDa according to human NP_112729.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 26kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

ABIN238598 (0.5µg/ml) staining of mouse heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Publikationen

Publikationen: Swanton, Marani, Pardo et al.: "Regulators of mitotic arrest and ceramide metabolism are determinants of sensitivity to paclitaxel and other chemotherapeutic drugs." in: Cancer cell, Vol. 11, Issue 6, pp. 498-512, 2007 (PubMed).