Abhydrolase Domain Containing 4 (ABHD4) (AA 304-316) Antikörper

Antigen: Abhydrolase Domain Containing 4 (ABHD4)

Synonyme: ABH4, FLJ12816, Abh4, AI429574, 1110035H23Rik, ABHD4, MGC137222, abhd5, zgc:110267

Epitope: AA 304-316

Klonalität: Polyklonal

Wirt: Ziege

Applikation: ELISA

Produktnummer: ABIN238584

Produktbeschreibung

Weitere Bezeichnung: ABHD4 (aa 304 to 316)

Gen-ID: 63874, 105501 (mouse)

Immunogen: C-QRPDSYVRDMEIK

Format: Affinity-purified

Beschreibung: All names and symbols: ABHD4, abhydrolase domain containing 4, FLJ12816. Official Symbol: ABHD4. RefSeq number(s): NP_071343.2

Spezifität: Human, Mouse, Rat, Dog

Anwendungen

Applikationshinweise: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 48kDa band in Human Brain (Cerebellum, Cerebellar Cortex, Frontal Cortex) lysates and in Mouse Brain lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 38.8kDa according to NP_071343.2. The 48kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Lagerung: Aliquot and store at -20°C. Minimize freezing and thawing.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Simon, Cravatt: "Endocannabinoid biosynthesis proceeding through glycerophospho-N-acyl ethanolamine and a role for alpha/beta-hydrolase 4 in this pathway." in: The Journal of biological chemistry, Vol. 281, Issue 36, pp. 26465-72, 2006 (PubMed).