Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Spezifität
The antibody MEM-G/2 recognizes the free heavy chain of all the HLA-G isoforms. HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on the surface of trophoblast cells.
Produktmerkmale
Synonyms: HLA-6.0, HLAG, MHC class I antigen G, HLA class I histocompatibility antigen
Aufreinigung
Protein A affinity chromatography
Immunogen
Bacterially expressed recombinant HLA-G heavy chain (denaturated).
Immunohistochemistry (paraffin sections). Western Blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS, pH 7.4 with 15 mM sodium azide as preservative
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Haltbarkeit
12 months
Target
HLAG
(HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG))
MHC-G antikoerper, B-F antikoerper, B-F-S04 antikoerper, B-F-S05 antikoerper, B-F-S06 antikoerper, B-F-S07 antikoerper, B-FI antikoerper, B-FIV antikoerper, BF2 antikoerper, BFa2 antikoerper, BFw-03 antikoerper, BFw-05 antikoerper, BFz-01 antikoerper, major histocompatibility complex, class I, G antikoerper, MHC BF1 class I antikoerper, HLA-G antikoerper, BF1 antikoerper
Hintergrund
HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.0, HLAG, MHC class I antigen G