Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) Antikörper

Antigen: Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1)

Synonyme: B1, S7, Bp35, CD20, MS4A2, LEU-16, MGC3969, Cd20, Ly-44, Ms4a2, AA960661, FcRB, Fce1b, Ms4a1, Fcer1b, Fcrbeta, FCIGA, MS4A1, bp35, cd20, ms4a2, leu-16, ms4a4c, cd20-like

Klonalität: Monoklonal (MEM-269)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN238472

Produktbeschreibung

Produktmerkmale: Synonyms: MS4A1, Leu-16, Bp35, B-cell marker, B-lymphocyte antigen CD20, Membrane-spanning4-domains subfamily A member 1, B-lymphocyte surface antigen B1

Weitere Bezeichnung: CD20

Swiss-Prot: P11836

Immunogen: RAMOS human lymphoma cell line.

Kreuzreaktivität: Human

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: MEM-269

Beschreibung: CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDanon-glycosylated surface phosphoprotein expressed on mature and most malignant Bcells, but not stem cells or plasma cells (low number of the CD20 has been also detectedon a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20regulates transmembrane calcium conductance (probably functioning as a component ofstore-operated calcium channel), cell cycle progression and B-cell proliferation. It isassociated with lipid rafts, but the intensity of this association depends on extracellulartriggering, employing CD20 conformational change and/or BCR (B cell antigen receptor)aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidlydissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 servesas a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressedat high levels on most B-cell malignancies, but does not become internalized or shed fromthe plasma membrane following mAb treatment.

Spezifität: The antibody MEM-269 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membranereceptor with four transmembrane domains, expressed on B lymphocytes (it is lost onplasma cells), follicular dendritic cells, and at low levels on peripheral blood Tlymphocytes. Species: Human.

Anwendungen

Applikationshinweise: Flow Cytometry. Immunohistochemistry (paraffin sections). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Purified

Buffer: PBS, pH 7.4 containing 15 mM sodium azide as preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Forschungsgebiet: Krebs, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, Adaptive Immunität, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar