Acrosomal Vesicle Protein 1 (ACRV1) Antikörper

Antigen: Acrosomal Vesicle Protein 1 (ACRV1)

Synonyme: SP-10, SPACA2, D11S4365, Sp10, ACRV1, Msa63

Klonalität: Monoklonal (Hs-36)

Wirt: Maus

Reaktivität: Human

Applikation: Immunfluoreszenz (IF), Western Blot (WB)

Produktnummer: ABIN238422

Produktbeschreibung

Produktmerkmale: Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein

Weitere Bezeichnung: ACRV1

Gen-ID: 56

Swiss-Prot: P26436

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.

Kreuzreaktivität: Human

Isotyp: IgM  (Passende Sekundärantikörper)

Klon: Hs-36

Beschreibung: One of the most frequent causes of man infertility is defective sperm acrosome. Thisdamage can be detected using antibodies against intra-acrosomal proteins. Besidesdiagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteinscan be used for evaluation of the physiological state of sperm cells as well as for selectionof a suitable method of fertilization in the laboratories of assisted reproduction.

Spezifität: The antibody Hs-36 reacts with human intra-acrosomal proteins (60kDa, 25kDa)associated with the membranes of the acrosomal vesicle. Species: Human. Others not tested.

Anwendungen

Applikationshinweise: Immunocytochemistry (10 μg/ml): Membrane permeabilization (acetone) is essential. Western Blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar