MHC Class I H-2Dk Antikörper

Antigen: MHC Class I H-2Dk

Klonalität: Monoklonal (H100-30-23)

Wirt: Maus

Reaktivität: Maus

Produktnummer: ABIN238410

Produktbeschreibung

Produktmerkmale: Synonyms: H-2D(K), H2-D1, H-2 class I histocompatibility antigen, D-K alpha chain

Weitere Bezeichnung: MHC Class I H2 Dk

Swiss-Prot: P14426

Kreuzreaktivität: Maus

Isotyp: IgG2b  (Passende Sekundärantikörper)

Klon: H100-30-23

Spezifität: This antibody is an MHC Class I antibody specific for cells expressing the H-2K and /or Dregion coded for by the k haplotype. It detects the H-2. m5 determinant (4). The reactionpattern of this antibody shows that it is also positive for H-2s(b,r,q) haplotypes. Species: Mouse. Others not tested. Add. Information: This reagent is not sold as sterile, but can be sterilized by filtration if necessary. Tominimize loss of volume during filtration, first dilute to the final working concentration inthe appropriate medium and then filter through a 0. 22 µ Millipore filter (or equivalent).

Anwendungen

Protokoll: RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF H-2k BEARINGLYMPHOCYTES: 1. Prepare a cell suspension from the appropriate tissue in Cedarlane Cytotoxicity Medium1or equivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on density cell separation medium. After washing, adjust the cellconcentration to 1x10e7 cells per ml in Cytotoxicity Medium.

Konzentration: 1x10e6 cells per mlComplement: Low toxicity Rabbit ComplementComplement 1: 15Procedure: Two stage cytotoxicity as described on page 3, Recommended Method forDetermining Percent of H-2k bearing cells in a Population.(see picture below)STRAIN DISTRIBUTION:

Reinigung: Ascites

Lagerung: Store the antibody (following reconstitution in aliquots) at -20 °C. Avoid repeated freezingand thawing. Shelf life: One year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar