»
Antikörper
»
anti-clathrin, Heavy Chain (Hc) (CLTC) Antikörper
clathrin, Heavy Chain (Hc) (CLTC) Antikörper
| Antigen |
|
| Synonyme |
Hc, CHC, CHC17, CLH-17, CLTCL2, KIAA0034, CLTC, Chc |
| Klonalität |
Monoklonal (BF-06) |
|
Wirt
|
|
|
Reaktivität
|
|
|
Applikation
|
 Alternativen Enzyme Immunoassay (EIA), Durchflusszytometrie (FACS), Immunfluoreszenz (IF), Immunpräzipitation (IP), Western Blot (WB)
|
| Produktnummer |
ABIN238388 |
| Menge |
0.1 mg (1.0 mg/ml) |
| Preis |
240,00 € Zzgl. Versandkosten €20,00 und MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
|
Produktmerkmale
|
Synonyms: CLTC, CLH17, CLTCL2, KIAA0034, Membrane Vesicle Marker, Clathrin heavy chain onchromosome 17
|
|
Weitere Bezeichnung
|
Clathrin heavy chain
|
|
Gen-ID
|
1213
|
|
Swiss-Prot
|
Q00610
|
|
Kreuzreaktivität
|
Rind (Kuh), Human, Maus, Schwein, Ratte (Rattus)
|
|
Isotyp
|
IgM (Passende Sekundärantikörper)
|
|
Klon
|
BF-06
|
|
Beschreibung
|
Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results inmembrane invagination. The basic oligomers are composed of three clathrin heavy chain(180 kDa) and three light chain (30 kDa) subunits and the process of polymerization isdynamically regulated by the light chains. Interaction of clathrin with the plasmamembrane is mediated by adaptor proteins (AP1-4) specific for different cellularcompartments. Another proteins, such as endophilin, epsin and amphiphysin are involvedin membrane invagination and clathrin rearrangements. Finally, dynamin functions at thefission stage of clathrin-mediated endocytosis.
|
|
Spezifität
|
The antibody recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa proteininvolved in receptor-mediated endocytosis. Species: Human, Porcine, Mouse, Rat, Bovine. Others not tested.
|
|
Applikationshinweise
|
Flow Cytometry: 2-5 ug/ml. Positive control: human blood leukocytes. Immunoprecipitation. Western Blotting. Immunocytochemistry. ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
|
|
Konzentration
|
1.0 mg/ml
|
|
Reinigung
|
Purified
|
|
Buffer
|
Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
|
|
Lagerung
|
Store the antibody at 2 - 8 °C. Do not freeze. Shelf life: one year from despatch.
|
|
Forschungsgebiet
|
Organelle, Transporter
|
|
Beschränkungen
|
Nur für Forschungszwecke einsetzbar
|
Alternativen zu Antigen "clathrin, Heavy Chain (Hc) (CLTC)", Typ "Antikörper" finden
|
Wirte
|
Maus (15), Kaninchen (2), Ziege (1)
|
|
Reaktivitäten
|
Human (11), Rind (Kuh) (6), Ratte (Rattus) (6), Maus (4), Schwein (2), Amphibien (1), Hamster (1), Primate (1)
|
|
Applikationen
|
Western Blot (WB) (17), Immunfluoreszenz (IF) (4), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), Immunpräzipitation (IP) (4), Enzyme Immunoassay (EIA) (3), Immunhistochemie (Paraffinschnitte) (IHC (p)) (3), ELISA (2), Durchflusszytometrie (FACS) (1), Immunzytochemie (ICC) (1)
|
