Biotin Antikörper (FITC)

Antigen: Biotin

Klonalität: Polyklonal

Wirt: Ziege

Konjugat: FITC

Applikation: Immunelektrophorese (IEP)

Produktnummer: ABIN236874

Produktbeschreibung

Immunogen: Biotin conjugated to Keyhole Limpet Hemocyanin (KLH).

Format: Lyophilized

Beschreibung: Goat anti Biotin Goat Antibody to Biotin Fluorescein conjugated

Spezifität: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Biotinylated IgG and Biotinylated Bovine Serum Albumin.

Anwendungen

Applikationshinweise: Specific methodologies have not been tested using this product.

Konzentration: 1mg/ml (OD280nm) (prior to lyophilization)

Reinigung: Immunoaffinity chromatography using Biotin coupled to sepharose beads. 4.6 moles FITC per mole of Goat IgG.

Buffer: Lyophilized from 0.02M Potassium phosphate, 0.15M Sodium Chloride, pH 7.2 containing 10mg/ml BSA (IgG and Protease free). Preservative: 0.01% Sodium azide

Lagerung: Store lyophilized product at 2-8°C. After reconstitution, product is stable for several weeks as an undiluted liquid. Prepare working dilution only prior to immediate use. For extended storage, reconstitute product with 50% glycerol instead of water and then aliquot and store at -20°C or below. Avoid multiple freeze/thaw cycles. Expiration is one year from date of reconstitution. : This product contains sodium azide, which has been classified as Xn (Harmful) in European Directive 67/548/EEC in the concentration range of 0.1-1.0%. When disposing of this reagent through lead or copper plumbing, flush with copious volumes of water to prevent azide build-up in drains.Reconstitute with 1ml of deionized water (or equivalent). Centrifuge product if not completely clear after standing at room temperature.

Forschungsgebiet: Signaltransduktion, Metabolismus, Epitop-Tags, Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: The, Feltkamp: "Conjugation of fluorescein isothiocyanate to antibodies. I. Experiments on the conditions of conjugation." in: Immunology, Vol. 18, Issue 6, pp. 865-73, 1970 (PubMed).