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Antikörper
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anti-Histone H1
Histone H1 Antikörper
| Antigen |
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| Synonyme |
H1c, H1.2, H1f2, His1a, H1var1, MGC107646, 0610008C09Rik, LOC692942, HISTONE H1, HON4 |
| Klonalität |
Monoklonal (AE-4) |
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Wirt
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| Produktnummer |
ABIN235436 |
| Menge |
2ml (Not determined) |
| Preis |
247,25 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferbar innerhalb von 5 bis 10 Werktagen |
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Format
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Supernatant, Liquid
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Isotyp
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IgG2a (Passende Sekundärantikörper)
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Klon
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AE-4
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Beschreibung
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MAb to Histone H1 Monoclonal Antibody to Histone H1
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Spezifität
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Recognizes histone H1 in the nucleus of animal and plant cells. Using purified histone fractions monoclonal AE-4 has been shown to have preferential binding to histone H1 by immunoblotting and ELISA methods.
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Applikationshinweise
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Produces a speckled staining pattern in the nucleus of normal and malignant cells and may be used to stain nuclear histones. We highly recommend fixing cells with 2.0% paraformaldehyde. Tonsil is the recommended positive control tissue. We suggest using this product without dilution. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
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Konzentration
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Not determined
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Reinigung
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Not applicable. Source: Tissue culture supernatant
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Buffer
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Filtered tissue culture supernatant (RPMI-1640), pH 7.2. Preservative: 0.09% Sodium azide
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Lagerung
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Short term (up to one month) store at 2-8°C. Long-term, aliquot and store below -20°C. Avoid multiple freeze/thaw cycles.
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Forschungsgebiet
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Chromatin und nukleäre Signaltransduktion, DNS/RNS, Transkriptionsfaktoren
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Histone H1", Typ "Antikörper" finden
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Wirte
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Kaninchen (28), Maus (21), Schaf (7), Huhn (5)
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Reaktivitäten
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Human (45), Rind (Kuh) (22), Maus (19), Ratte (Rattus) (16), Hund (15), Pferd (15), Schwein (15), Kaninchen (15), Säugetiere (2)
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Applikationen
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Western Blot (WB) (38), Immunfluoreszenz (IF) (37), ELISA (21), Immunhistochemie (Paraffinschnitte) (IHC (p)) (19), Durchflusszytometrie (FACS) (13), Immunpräzipitation (IP) (11), Immunhistochemie (IHC) (10), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (8), Enzyme Immunoassay (EIA) (4), Immunzytochemie (ICC) (4), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Chromatin-Immunopräzipitation (ChiP) (1), Immunelektronenmikroskopie (EM) (1)
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Konjugate
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Alexa Flour 488 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1)
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Epitope
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Acetyl-Lys25 (2), AA 100-200 (1), C-Term (1), N-Term (1), pThr17 (1)
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Alternativen 
