8-Hydroxyguanosine (8-OHDG) Antikörper

Antigen: 8-Hydroxyguanosine (8-OHDG)

Klonalität: Monoklonal (15A3)

Wirt: Maus

Applikation: ELISA, Immunhistochemie (Paraffinschnitte) (IHC (p))

Produktnummer: ABIN234190

Produktbeschreibung

Weitere Bezeichnung: 8-Hydroxyguanosine

Immunogen: 8-hydroxyguanosine (oh8G)-BSA and ?casein conjugates

Format: Purified, Liquid

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: 15A3

Beschreibung: MAb to 8-Hydroxyguanosine Monoclonal Antibody to 8-Hydroxyguanosine

Spezifität: Recognizes 8-hydroxy-2?-deoxyguanosine (oh8dG), 8-hydroxyguanine (oh8G), and 8-hydroxyguanosine (oh8G). These are markers of oxidative damage to DNA and RNA.

Anwendungen

Applikationshinweise: Suitable for use in ELISA and immunohistochemistry (PLP-fixed sections) to detect the markers of oxidative damage to DNA and RNA. May also be used on immunoaffinity columns to isolate oxidative DNA damage adducts from biological fluids. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Konzentration: 1mg/ml (OD280nm)

Reinigung: Protein G chromatography. Source: Ascites

Buffer: TBS, pH 7.4. Preservative: 0.1% Sodium azide

Lagerung: Store at 2-8°C. DO NOT FREEZE!

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Park, Shigenaga, Degan et al.: "Assay of excised oxidative DNA lesions: isolation of 8-oxoguanine and its nucleoside derivatives from biological fluids with a monoclonal antibody column." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 8, pp. 3375-9, 1992 (PubMed).

Nunomura, Perry, Pappolla et al.: "RNA oxidation is a prominent feature of vulnerable neurons in Alzheimer's disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 6, pp. 1959-64, 1999 (PubMed).

Cui, Holmes, Liu: "Oxidative damage to the c-fos gene and reduction of its transcription after focal cerebral ischemia." in: Journal of neurochemistry, Vol. 73, Issue 3, pp. 1164-74, 1999 (PubMed).

Salganik, Albright, Rodgers et al.: "Dietary antioxidant depletion: enhancement of tumor apoptosis and inhibition of brain tumor growth in transgenic mice." in: Carcinogenesis, Vol. 21, Issue 5, pp. 909-14, 2000 (PubMed).