Proteine Tyrosine Phosphatase (IA-2) Antikörper

Antigen: Proteine Tyrosine Phosphatase (IA-2)

Klonalität: Monoklonal (103-2C4)

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Immunfluoreszenz (IF), Immunpräzipitation (IP), Radioimmunoassay (RIA), Western Blot (WB)

Produktnummer: ABIN233871

Produktbeschreibung

Immunogen: Human recombinant IA-2ic

Format: Affinity-purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 103-2C4

Spezifität: Epitope region of IA-2: aa 771-979

Anwendungen

Applikationshinweise: Suitable for the detection of non-insulin containing beta cells. The antibody performed well on formalin fixed, paraffin embedded sections from human, mouse and rat pancreas

Konzentration: 100µg/200µl

Reinigung: Affinity purified mouse IgG (liquid, buffer: PBS/0.9mg/ml sodium azid)

Lagerung: at -20°C

Forschungsgebiet: Diabetes

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Striking binding of mc-IA-2-Ab as ICA on cryosections of human (A) and mouse (B) pancreas measured by indirect immunofluorescence.

IA-2 (intracellulare domain) was separated by SDS-PAGE according to Laemmli with 1.2µg IA-2 per antibody. The strips of the membrane were incubated with the monoclonal antibodies (0.1mg/0.2ml, diluted 1:100). Binding of the monoclonal antibodies was detected with Peroxidase-conjugated Rabbit Anti-Mouse IgG, Fc (gamma) Fragment and 3,3‘-Diaminobenzidin tetrahydrochlorid was used as chromogen.