Cullin-associated and Neddylation Dissociated (CAND1) (N-Term specific) Antikörper

Antigen: Cullin-associated and Neddylation Dissociated (CAND1)

Synonyme: ATCAND1, CAND1, CULLIN-ASSOCIATED AND NEDDYLATION DISSOCIATED, CULLIN-ASSOCIATED AND NEDDYLATION DISSOCIATED 1, ETA2, HEMIVENATA, HVE, T8K22.14, T8K22_14, TIP120

Epitope: N-Term specific

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN233780

Produktbeschreibung

Weitere Bezeichnung: CAND1 (N-terminal specific)

Immunogen: This antibody was prepared from whole rabbit serum produced by repeated immunizations with a syntheticpeptide corresponding to amino acids 11-24 of Human CAND1/TIP120A (N-terminal) coupled to KLH. Protein Sequence: Human CAND1 (TIP120A) (1230 aa 136.4 kDa) 1 masasyhisn llekmtssdk dfrf matndl mtelqkdsik ldddserkvv kmilklledk 61 ngevqnlavk clgplvskvk eyqvetivdt lctnmlsdke qlrdissigl ktvigelppa 121 ssgsalaanv ckkitgrlts aiakqedvsv qlealdimad mlsrqggllv nfhpsiltcl 181 lpqltsprla vrkrtiialg hlvmscgniv fvdliehlls elskndsmst trtyiqciaa 241 isrqaghrig eylekiiplv vkfcnvddde lreyciqafe sfvrrcpkev yphvstiini … 901 sqpkrqylll hslkeiissa svvglkpyve niwalllkhc ecaeegtrnv vaeclgkltl 961 idpetllprl kgylisgssy arssvvtavk ftisdhpqpi dpllkncigd flktledpdl 1021 nvrrvalvtf nsaahnkpsl irdlldtvlp hlynetkvrk elirevemgp fkhtvddgld 1081 irkaafecmy tlldscldrl difeflnhve dglkdhydik mltflmlvrl stlcpsavlq 1141 rldrlveplr atcttkvkan svkqefekqd elkrsamrav aalltipeae ksplmsefqs 1201 qissnpelaa ifesiqkdss stnlesmdts

Format: Liquid (sterile filtered)

Beschreibung: CAND1 is also known as TIP120A, and TATA-binding protein-interacting protein 120A. The SCFcomplex consists of the invariable components Skp1, Cul1, and Rbx1 as well as a variable F-box protein, and functions as anE3 ubiquitin ligase. E3 ubiquitin ligases regulate various physiological processes. CAND1 binds to Cul1 and potentiallyregulates the SCF complex. CAND1 physically associates with Cul1 in the nucleus and this interaction is mediated by a centralregion of Cul1 distinct from its binding sites for Skp1 and Rbx1. CAND1 selectively binds to unneddylated CUL1 and isdissociated by CUL1 neddylation. CAND1 forms a ternary complex with CUL1 and ROC1.

Spezifität: This product is monospecific antiserum processed by delipidation and defibrination followed by sterilefiltration. This product reacts with human, rat and mouse CAND1/TIP120A. Cross reactivity does occur with human, rat andmouse CAND2/TIP120B. Cross reactivity with CAND1 from other sources is not known.

Anwendungen

Applikationshinweise: Recommended Dilutions: ELISA 1:2,000 - 1:10,000 WESTERN BLOT 1:500 - 1:1,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This antibody reacts with human, rat and mouse CAND1 by western blot and immunoprecipita-tion.The antibody immunoprecipitates in vitro translated protein andprotein from cell lysates (using HeLa and NIH-3T3, andothers). Coimmunoprecipitation of related proteins has notbeen tested. A 136.4 kDa band corresponding to humanCAND1 is detected. Most cell lines expressing CAND1 can beused as a positive control. Researchers should determineoptimal titers for other applications.

Konzentration: 85 mg/ml (by Refractometry)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: None.

Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extendedstorage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date isone (1) year from date of opening.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a