Heat Shock 70 KDa (HSP70) Antikörper

Antigen: Heat Shock 70 KDa (HSP70)

Synonyme: BIP, MIF2, GRP78, FLJ26106, Bip, Sez7, mBiP, Grp78, SEZ-7, Hsce70, AL022860, AU019543, D2Wsu17e, D2Wsu141e, hspa5a

Klonalität: Monoklonal

Wirt: Maus

Reaktivität: Human, Maus, Ratte (Rattus), Schaf

Applikation: Immunhistochemie (IHC), ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN233748

Produktbeschreibung

Weitere Bezeichnung: Hsp70

Gen-ID: NP_005337

Swiss-Prot: Q5JQI4

Immunogen: This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide from the region of amino acid residues 436-503 of human Hsp70 protein.

Format: Liquid (sterile filtered)

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Hsp70 genes encode abundant heat- inducible 70-kDa hsps (hsp70s). In most eukaryotes, hsp70 genes exist as part of a multigene family. Hsp70s are found in most cellular compartments of eukaryotes, including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (2). The N-terminal two-thirds of hsp70s are more conserved than the C-terminal one-third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins, preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization, and protein transport.

Spezifität: This Protein G purified monoclonal antibody reacts with human Hsp70 protein. A BLAST analysis was used to suggest cross-reactivity with Hsp70 from human, bovine, mouse, rat, C.elegans, dog, chicken, Drosophila, carp, guinea pig, hamster, monkey, pig, rabbit and sheep sources based on 100% homology with the immunizing sequence. No cross-reactivity occurs with hsc70 (hsp73). Cross-reactivity with Hsp70 from other sources has not been determined.

Anwendungen

Applikationshinweise: This Protein G purified antibody has been tested for use in ELISA, immunofluorescence microscopy, western blotting, FACS, immunoelectron microscopy, immunohistochemistry and immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 70 kDa in size corresponding to Hsp70 by western blotting in the appropriate cell lysate or extract. In general, a 1:1,000 dilution is suggested for most applications and is suitable to detect Hsp70 in 20 u g of heat shocked HeLa cell lysate by western blotting.

Konzentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: 50% (v/v) Glycerol. Preservative: 0.09% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Lagerung: Store vial at -20° C or below prior to opening. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Krebs, Hitzeschockproteine, Signaltransduktion

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot using antibodies-online's Protein G purified anti-Hsp70 antibody shows detection of Hsp70 in whole cell lysates from heat shocked HeLa cells. The band marked by the arrowhead corresponds to Hsp70 at an approximate molecular weight of 70 kDa. The primary antibody was used at a 1:1000 dilution.