CD207 Molecule, Langerin (CD207) Antikörper

Antigen: CD207 Molecule, Langerin (CD207)

Synonyme: CD207, CLEC4K

Klonalität: Monoklonal (DCGM4-122D5-03)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Durchflusszytometrie (FACS), Western Blot (WB)

Produktnummer: ABIN223764

Produktbeschreibung

Weitere Bezeichnung: CD207 / Langerin

Immunogen: In vitro-derived human DCs from CD34+ cells in GM-CSF+TNFalpha

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: DCGM4-122D5-03

Beschreibung: Langerin/CD207 is a transmembrane C-type lectin receptor (CLR) of epidermal and mucosal Langerhans cells (LCs) that induces Birbeck granule formation. Langerin features a single carbohydrate recognition domain (CRD) with mannose-type specificity in its extracellular portion. Langerin is unique among the CLRs in that it contains an intracellular domain with a proline-rich motif. Langerin expression has not been reported outside the DC system. (Valladeau J et al, 2000 Immunity, 12 : 71-81)

Spezifität: Human Langerin (extracellular epitope in Carbohydrate Recognition domain)

Anwendungen

Applikationshinweise: This monoclonal antibody has been studied antigen binding (competes with mannan), endocytosis, IHC, FACS, WB Usage recommendation: *This monoclonal antibody may be used between 5-20 ug/ml *Optimal dilution should be determined y each laboratory for each application *Coupled antibody: to maintain RT before using Aliquot storage conditions -20°C. KEEP CONTENTS STERILE: no preservative. Purified antibodies: avoid repeated freeze/thaw cycles. Coupled antibodies: glycerol protects from freezing .. Usage Recommendation: This monoclonal antibody may be used between 5-20 ug/ml .

Buffer: 100 ug in 200ul Tris-NaCl pH 8

Lagerung: -20°C. KEEP CONTENTS STERILE: no preservative.

Forschungsgebiet: CD Antigene

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

DCGM4-Langerin transfected fibroblasts

Dendritic cells in vitro

anti-CD207 Molecule, Langerin (CD207) antibody (Image 3)

Publikationen

Publikationen: Schuster, Vaculik, Fiala et al.: "HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells." in: The Journal of experimental medicine, Vol. 206, Issue 1, pp. 169-81, 2009 (PubMed).