GFP Antikörper (Green Fluorescent Protein)

Details for Product anti-GFP Antibody No. ABIN223515, Anbieter: Anmelden zum Anzeigen
Antigen
  • green fluorescent protein
  • gfp
Reaktivität
Aequorea victoria
638
16
5
5
2
2
Wirt
Kaninchen
340
170
80
40
16
9
9
2
Klonalität
Polyklonal
Konjugat
Dieser GFP Antikörper ist unkonjugiert
33
31
28
14
12
12
12
10
9
9
8
8
7
6
6
6
6
6
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Immunoprecipitation (IP), Western Blotting (WB)
536
354
202
156
144
134
96
87
73
25
15
13
13
8
8
6
6
5
4
4
3
3
3
3
3
2
2
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Recombinant EGFP
Isotyp IgG
Spezifität The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc.
Reinigung Affinity purified
Andere Bezeichnung GFP
Hintergrund Green fluorescent protein (GFP) is a spontaneously fluorescent protein isolated from pacific jellyfish, Aequorea victoria. It transduces the blue chemiluminescence into green fluorescent light. Since the molecular cloning of GFP cDNA and demonstration of GFP as a functional transgene, GFP has become a powerful tool with exciting applications in developmental, cell and molecular biology. GFP fluorescence is not species specific and can be expressed in bacteria, yeast, plant and mammalian cells. GFP can fuse with proteins of interest without interfering significantly with their assembly and function.
Synonyms: Green Fluorescent Protein
Gen-ID 110612126
Forschungsgebiet Tags/Labels
Applikationshinweise Western blotting (0.5-4 μg/mL) and in immunoprecipitation (10-20 μg/mL). However, the optimal conditions should be determined individually. The antibody reacts with GFP, and its variants EGFP, RFP, YFP, and CFP, etc. Recombinant EGFP, RFP, YFP and CFP can be used as positive controls.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.2 mg/mL
Buffer 100 μg (0.2 mg/mL) affinity purified rabbit anti-GFP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung The antibody solution should be gently mixed before use.
Lagerung -20 °C
Informationen zur Lagerung For long-term storage, aliquot and freeze at -70 °C.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-Green Fluorescent Protein (GFP) antibody (ABIN223515) Western blot analysis of GFP antibody using recombinant RFP (dsRed, Lane 1 & 2), YFP ...
Produkt verwendet in: Maus, Jairaman, Stathopulos, Muik, Fahrner, Weidinger, Benson, Fuchs, Ehl, Romanin, Ikura, Prakriya, Feske: "Missense mutation in immunodeficient patients shows the multifunctional roles of coiled-coil domain 3 (CC3) in STIM1 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, Issue 19, pp. 6206-11, 2015 (PubMed).

German, Mammoto, Jiang, Ingber, Mammoto: "Paxillin controls endothelial cell migration and tumor angiogenesis by altering neuropilin 2 expression." in: Journal of cell science, Vol. 127, Issue Pt 8, pp. 1672-83, 2014 (PubMed).

Hagen, Bayer, Rösch, Schindler: "The intraviral protein interaction network of hepatitis C virus." in: Molecular & cellular proteomics : MCP, Vol. 13, Issue 7, pp. 1676-89, 2014 (PubMed).

Hsu, Lin, Chang: "Synergetic regulation of translational reading-frame switch by ligand-responsive RNAs in mammalian cells." in: Nucleic acids research, Vol. 42, Issue 22, pp. 14070-82, 2014 (PubMed).

Martínez-Fábregas, Díaz-Moreno, González-Arzola, Janocha, Navarro, Hervás, Bernhardt, Velázquez-Campoy, Díaz-Quintana, De la Rosa: "Structural and functional analysis of novel human cytochrome C targets in apoptosis." in: Molecular & cellular proteomics : MCP, Vol. 13, Issue 6, pp. 1439-56, 2014 (PubMed).

Mellergaard, Skovbakke, Schneider, Lauridsen, Andresen, Jensen, Skov: "N-glycosylation of asparagine 8 regulates surface expression of major histocompatibility complex class I chain-related protein A (MICA) alleles dependent on threonine 24." in: The Journal of biological chemistry, Vol. 289, Issue 29, pp. 20078-91, 2014 (PubMed).

Martínez-Fábregas, Díaz-Moreno, González-Arzola, Janocha, Navarro, Hervás, Bernhardt, Díaz-Quintana, De la Rosa: "New Arabidopsis thaliana cytochrome c partners: a look into the elusive role of cytochrome c in programmed cell death in plants." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 12, pp. 3666-76, 2013 (PubMed).

Makovski, Yaffe, Shpungin, Nir: "Intronic promoter drives the BORIS-regulated expression of FerT in colon carcinoma cells." in: The Journal of biological chemistry, Vol. 287, Issue 9, pp. 6100-12, 2012 (PubMed).

Andresen, Skovbakke, Persson, Hagemann-Jensen, Hansen, Jensen, Skov: "2-deoxy D-glucose prevents cell surface expression of NKG2D ligands through inhibition of N-linked glycosylation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 188, Issue 4, pp. 1847-55, 2012 (PubMed).

Bhat, Folimonova, Cole, Ballard, Lei, Watson, Sumner, Nelson: "Influence of host chloroplast proteins on Tobacco mosaic virus accumulation and intercellular movement." in: Plant physiology, Vol. 161, Issue 1, pp. 134-47, 2012 (PubMed).

Welman, Serrels, Brunton, Ditzel, Frame: "Two-color photoactivatable probe for selective tracking of proteins and cells." in: The Journal of biological chemistry, Vol. 285, Issue 15, pp. 11607-16, 2010 (PubMed).

Kock, Bulgakova, Knust, Sinning, Panneels: "Targeting of Drosophila rhodopsin requires helix 8 but not the distal C-terminus." in: PLoS ONE, Vol. 4, Issue 7, pp. e6101, 2009 (PubMed).

Creighton, Zhu, Alexeyev, Stevens: "Spectrin-anchored phosphodiesterase 4D4 restricts cAMP from disrupting microtubules and inducing endothelial cell gap formation." in: Journal of cell science, Vol. 121, Issue Pt 1, pp. 110-9, 2007 (PubMed).

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