GFP Antikörper

Antigen: GFP

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Kaninchen

Applikation: Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN223515

Produktbeschreibung

Gen-ID: 110612126

Immunogen: Recombinant EGFP (Internal ID# BV-315)

Format: Purified

Beschreibung: 100 ug purified rabbit polyclonal antibody. Recognizes wild-type GFP and its derivatives such as EGFP/EBFP etc. Performs well in Western blot analysis and immunoprecipitation. Synonyms: GFP Polyclonal Antibody, Anti-GFP antibody, GFP antibody, Anti-GFP, GFP, green fluorescence protein, EGFP, EBFP, EYFP, RFP Synonyms: GFP Polyclonal Antibody, Anti-GFP antibody, GFP antibody, Anti-GFP, GFP, green fluorescence protein, EGFP, EBFP, EYFP, RFP

Anwendungen

Applikationshinweise: The antibody can be used for Western blotting (0.5-4 ug/ml). Based on researcher's feedback, it can also be used in immunoprecipitation (10-20 ug/ml). However, the optimal conditions should be determined individually.

Buffer: 100 ug (0.2 mg/ml) affinity purified rabbit anti-GFP polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.

Lagerung: Store at -20 o C. For long-term storage, aliquot and freeze at -70 o C. Avoid repeated freeze/defrost cycles Shelf Life: 12 Months

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis of GFP antibody using recombinant RFP (dsRed, Lane 1 & 2), YFP (Lane 3 & 4) and EGFP (Lane 5 & 6).

Publikationen

Publikationen: Creighton, Zhu, Alexeyev et al.: "Spectrin-anchored phosphodiesterase 4D4 restricts cAMP from disrupting microtubules and inducing endothelial cell gap formation." in: Journal of cell science, Vol. 121, Issue Pt 1, pp. 110-9, 2007 (PubMed).

Kock, Bulgakova, Knust et al.: "Targeting of Drosophila rhodopsin requires helix 8 but not the distal C-terminus." in: PLoS ONE, Vol. 4, Issue 7, pp. e6101, 2009 (PubMed).

Welman, Serrels, Brunton et al.: "Two-color photoactivatable probe for selective tracking of proteins and cells." in: The Journal of biological chemistry, Vol. 285, Issue 15, pp. 11607-16, 2010 (PubMed).