Myc Tag Antikörper

Details zu Produkt Nr. ABIN223509, Anbieter: Anmelden zum Anzeigen
Antigen
  • MRTL
  • MYCC
  • bHLHe39
  • c-Myc
  • MYC proto-oncogene, bHLH transcription factor
  • MYC
Reaktivität
Tag
216
41
14
12
6
5
3
1
1
Wirt
Kaninchen
163
63
21
17
3
2
1
Klonalität
Polyklonal
Konjugat
Unkonjugiert
28
24
15
9
5
4
4
4
4
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
226
121
81
52
52
39
23
19
19
7
6
5
5
5
5
4
3
3
3
2
1
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Synthetic peptide corresponding to residues surrounding amino acid 413 of human c-Myc
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN412841.
Sequenz EQKLISEEDL
Isotyp IgG
Spezifität The antibody detects Myc-Tag proteins overexpressed in cells.
It does not detect endogenous levels of the transcription factor c-Myc.
Reinigung Affinity purified
Andere Bezeichnung C-Myc Epitope Tag
Substanzklasse Tag
Hintergrund Epitope tags are usually for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The Myc epitope tag is widely used to detect expression of recombinant proteins in bacteria, yeast, insect and mammalian systems.
Synonyms: MYC , C-MYC , c-Myc
Gen-ID 4609
UniProt P01106
Forschungsgebiet Transcription Factors
Applikationshinweise Western blotting (1-2 μg/mL), immunoprecipitation (20-40 μg/mL), and immunocytochemistry (10-20 μg/mL). However, the optimal conditions should be determined individually. The antibody detects Myc-Tag proteins overexpressed in cells. It does not detect endogenous levels of the transcription factor c-Myc.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) peptide affinity purified rabbit anti-Myc-Tag polyclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal.
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung The antibody solution should be gently mixed before use.
Lagerung -20 °C
Informationen zur Lagerung For long-term storage, aliquot and freeze at -70 °C.
Haltbarkeit 12 months
Bilder des Herstellers
 image for anti-Myc Tag antibody (ABIN223509) anti-Myc Tag antibody
Produkt verwendet in: Kumar, Shum, Glaunsinger: "Importin alpha-mediated nuclear import of cytoplasmic poly(A) binding protein occurs as a direct consequence of cytoplasmic mRNA depletion." in: Molecular and cellular biology, Vol. 31, Issue 15, pp. 3113-25, 2011 (PubMed).

Lei, Shi, Basu, Huq, Routhier, Day, Jin: "Proteolytic processing of angiopoietin-like protein 4 by proprotein convertases modulates its inhibitory effects on lipoprotein lipase activity." in: The Journal of biological chemistry, Vol. 286, Issue 18, pp. 15747-56, 2011 (PubMed).

Liu, Afroza, Rader, Jin: "Angiopoietin-like protein 3 inhibits lipoprotein lipase activity through enhancing its cleavage by proprotein convertases." in: The Journal of biological chemistry, Vol. 285, Issue 36, pp. 27561-70, 2010 (PubMed).

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