PCNA Antikörper
| Antigen |
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| Klonalität |
Polyklonal |
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Wirt
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Reaktivität
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Human (190), Ratte (Rattus) (110), Maus (97), Rind (Kuh) (41), Hund (41), Kaninchen (36), Huhn (21), Diverse Spezies (10), Hefe (Saccharomyces Cerevisiae) (7), Fruchtfliege (Drosophila melanogaster) (6), Pferd (6), Yeast (Pichia pastoris) (6), Fisch (5), Säugetiere (5), Schwein (5), Wirbeltiere (Vertebraten) (5), Amphibien (4), Insekten (4), Affe (2), Xenopus laevis (2), Frosch (1), Primate (1), Schizosaccharomyces pombe (S. pombe) (1), Schaf (1)
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Applikation
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 Alternativen Western Blot (WB), Immunpräzipitation (IP), Immunhistochemie (IHC)
Western Blot (WB) (137), Durchflusszytometrie (FACS) (86), Immunhistochemie (Paraffinschnitte) (IHC (p)) (82), Immunfluoreszenz (IF) (78), Immunpräzipitation (IP) (48), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (28), ELISA (22), Immunhistochemie (IHC) (20), Enzyme Immunoassay (EIA) (17), Intrazelluläre Durchflusszytometrie (ICFC) (10), Immunzytochemie (ICC) (6), ELISA (Detektionsantikörper) (2), Immunoelectron Microscopy (IEM) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1)
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| Produktnummer |
ABIN223074 |
| Menge |
100 ug |
| Preis |
276,63 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 7 bis 10 Werktagen |
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Gen-ID
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5111
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Immunogen
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Synthetic peptide corresponding to the C-terminus of human PCNA (Internal ID# BV-962).
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Format
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Purified
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Beschreibung
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100 ug purified rabbit polyclonal antibody. Reacts with human/mouse/rat. Tested in WB/IP/IHC.
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Applikationshinweise
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The antibody can be used for Western blotting (0.5-4 ug/ml). Per researcher's feedback, it can also be used in immunoprecipitation and immunohistochemistry (frozen & paraffin). However, the optimal conditions should be determined individually. Blocking peptide is also available separately.
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Buffer
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100 ug (0.2 mg/ml) affinity purified rabbit anti-PCNA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
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Lagerung
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Store at -20 o C. For long-term storage, aliquot and freeze at -70 o C. Avoid repeated freeze/defrost cycles Shelf Life: 12 Months
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Forschungsgebiet
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Proliferationsmarker
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Publikationen
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Lyzogubov, Tytarenko, Thotakura et al.: "Inhibition of new vessel growth in mouse model of laser-induced choroidal neovascularization by adiponectin peptide II." in: Cell biology international, Vol. 33, Issue 7, pp. 765-71, 2009 (PubMed).
Bora, Jha, Lyzogubov et al.: "Recombinant membrane-targeted form of CD59 inhibits the growth of choroidal neovascular complex in mice." in: The Journal of biological chemistry, Vol. 285, Issue 44, pp. 33826-33, 2010 (PubMed).
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Alternativen zu Antigen "PCNA", Typ "Antikörper" finden
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Wirte
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Maus (138), Kaninchen (97), Ziege (1)
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Reaktivitäten
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Human (190), Ratte (Rattus) (110), Maus (97), Rind (Kuh) (41), Hund (41), Kaninchen (36), Huhn (21), Diverse Spezies (10), Hefe (Saccharomyces Cerevisiae) (7), Fruchtfliege (Drosophila melanogaster) (6), Pferd (6), Yeast (Pichia pastoris) (6), Fisch (5), Säugetiere (5), Schwein (5), Wirbeltiere (Vertebraten) (5), Amphibien (4), Insekten (4), Affe (2), Xenopus laevis (2), Frosch (1), Primate (1), Schizosaccharomyces pombe (S. pombe) (1), Schaf (1)
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Applikationen
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Western Blot (WB) (137), Durchflusszytometrie (FACS) (86), Immunhistochemie (Paraffinschnitte) (IHC (p)) (82), Immunfluoreszenz (IF) (78), Immunpräzipitation (IP) (48), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (28), ELISA (22), Immunhistochemie (IHC) (20), Enzyme Immunoassay (EIA) (17), Intrazelluläre Durchflusszytometrie (ICFC) (10), Immunzytochemie (ICC) (6), ELISA (Detektionsantikörper) (2), Immunoelectron Microscopy (IEM) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Dot Blot (Dot) (1)
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Konjugate
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FITC (19), Biotin (14), Alexa Fluor 488 (7), Alexa Fluor 647 (7), PE (6), Alexa Fluor 350 (5), Alexa Fluor 555 (5), PE,Cy5.5 (5), Alexa Flour 488 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), Gold (2), HRP (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), RBITC (2), APC (1), RPE (1)
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Epitope
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AA 75-125 (1)
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