Caspase 8 Antikörper (Caspase 8, Apoptosis-Related Cysteine Peptidase) (active)

Details for Product anti-CASP8 Antibody No. ABIN223020, Anbieter: Anmelden zum Anzeigen
Antigen
  • casp8-A
  • xCaspase-8
  • casp8
  • CASP8
  • CASP-8
  • FLICE
  • MACH
  • Mch5
  • ALPS2B
  • CAP4
  • Casp-8
  • MCH5
  • caspase-8
  • zgc:92075
  • caspase 8 L homeolog
  • death related ced-3/Nedd2-like protein
  • caspase 8
  • caspase-8
  • caspase-8-like
  • caspase 8, apoptosis-related cysteine peptidase
  • casp8.L
  • LOC661095
  • CASP8
  • LOC5563818
  • casp8
  • LOC100222284
  • Casp8
Epitop
active
43
19
18
16
15
12
10
10
8
8
7
6
6
6
6
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Maus, Ratte (Rattus)
361
120
95
25
21
20
3
2
1
Wirt
Kaninchen
278
115
10
2
Klonalität
Polyklonal
Biologische Aktivität
Active
Konjugat
Dieser Caspase 8 Antikörper ist unkonjugiert
18
13
8
4
4
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Applikation
Western Blotting (WB)
360
109
92
79
51
36
36
29
14
13
9
8
6
5
2
2
2
1
1
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Synthetic peptide corresponding to residues surrounding amino acid 392 of mouse caspase-8
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN412658.
Isotyp IgG
Spezifität The antibody detects the 25 kDa large subunit of caspase-8.
It does not detect the full-length of caspase-8.
Reinigung Affinity purified
Andere Bezeichnung Caspase-8 (CASP8 Antibody Abstract)
Hintergrund Caspase-8, a member of the caspase-family of proteases, plays a key role in mediating Fas (CD95) and TNF induced apoptosis. Caspase-8 is synthesized as inactive pro-enzyme and activation of the enzyme involves proteolytic cleavage that leads to the release of the active p18 and p10 subunits. Activated caspase-8 is able to cleave and activate downstream caspases, such as caspase-3, -6, -7 and a death agonist member of the Bcl-2/Bcl-xL family, Bid, leading to apoptosis.
Synonyms: CASP8 , MGC78473 , CASP-8, MACH, procaspase-8, MCH5, ALPS2B, FLICE, CAP4, EC 3.4.22.61
Gen-ID 12370
Forschungsgebiet Apoptosis/Necrosis
Pathways Apoptose, Caspase Kaskade in der Apoptose, TLR Signalweg, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades
Applikationshinweise Western blotting (0.5-4 μg/mL) in samples from mouse and rat origins. However, the optimal conditions should be determined individually. The antibody detects the 25 kDa large subunit of caspase-8. It does not detect the full-length of caspase-8.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit polyclonal antibody in PBS containing 50 % glycerol, 0.5 % BSA, and 0.01 % thimerosal.
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung The antibody solution should be gently mixed before use.
Lagerung -20 °C
Informationen zur Lagerung For long-term storage, aliquot and freeze at -70 °C.
Haltbarkeit 12 months
Bilder des Herstellers
Western Blotting (WB) image for anti-Caspase 8, Apoptosis-Related Cysteine Peptidase (CASP8) (active) antibody (ABIN223020) Western blot analysis of caspase-8 expression in mouse intestine and rat kidney tissu...
Produkt verwendet in: Takano, Mihira, Fujioka, Hosoki, Chishti, Saido: "Vital role of the calpain-calpastatin system for placental-integrity-dependent embryonic survival." in: Molecular and cellular biology, Vol. 31, Issue 19, pp. 4097-106, 2011 (PubMed).

Pattison, Sanbe, Maloyan, Osinska, Klevitsky, Robbins: "Cardiomyocyte expression of a polyglutamine preamyloid oligomer causes heart failure." in: Circulation, Vol. 117, Issue 21, pp. 2743-51, 2008 (PubMed).

Mahul-Mellier, Strappazzon, Petiot, Chatellard-Causse, Torch, Blot, Freeman, Kuhn, Garin, Verna, Fraboulet, Sadoul: "Alix and ALG-2 are involved in tumor necrosis factor receptor 1-induced cell death." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34954-65, 2008 (PubMed).

Woods, Burns, Bradford, Ross, Kosyk, Swenberg, Cunningham, Rusyn: "WY-14,643 induced cell proliferation and oxidative stress in mouse liver are independent of NADPH oxidase." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 98, Issue 2, pp. 366-74, 2007 (PubMed).

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