CD20 Antikörper (Membrane-Spanning 4-Domains, Subfamily A, Member 1) (APC)

Details for Product anti-MS4A1 Antibody No. ABIN2214511, Anbieter: Anmelden zum Anzeigen
Antigen
  • MS4A1
  • bp35
  • cd20
  • ms4a2
  • leu-16
  • ms4a4c
  • cd20-like
  • B1
  • Bp35
  • CD20
  • CVID5
  • LEU-16
  • MS4A2
  • S7
  • AA960661
  • Cd20
  • Ly-44
  • Ms4a2
  • membrane-spanning 4-domains, subfamily A, member 2
  • membrane-spanning 4-domains, subfamily A, member 1
  • MS4A2
  • MS4A1
  • ms4a1
  • LOC100357719
  • Ms4a1
Reaktivität
Human
925
79
52
35
23
22
17
13
7
7
5
3
2
2
Wirt
Kaninchen
708
196
24
21
1
1
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD20 Antikörper ist konjugiert mit APC
71
70
48
37
22
18
18
17
15
14
12
10
10
10
10
9
8
7
7
7
6
6
5
4
4
4
4
4
4
3
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS)
642
202
185
135
130
124
116
97
49
20
14
14
13
9
8
7
5
4
4
2
2
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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'Independent Validation' Siegel
Chargennummer HG08OC1306
Validierte Anwendung Flow Cytometry
Positivkontrolle peripheral blood mononuclear cells
Negativkontrolle peripheral blood mononuclear cells
Bewertung None
Primärantikörper
Kontrollen
  • Positive control: peripheral blood mononuclear cells stained with anti-CD20
  • Negative control: peripheral blood mononuclear cells that are CD20 negative and unstained cells
  • Isotype Control: None
  • Secondary Only control: None
Protokoll
  • 1. 10mls of blood (collected in EDTA) was diluted to 20mls with PBS + 0.5% BSA
  • 2. 10mls of diluted blood was overlaid onto 5mls of ficoll.
  • 3. Spin for 20 minutes at 1500 rpm with break off.
  • 4. The buffy coat layer was collected and wash with PBS/BSA.
  • 5. Residual red blood cells were lysed by resuspending the cells in 1ml ammonium chloride for 5-10 minutes at room temperature.
  • 6. The cells were washed with PBS/BSA, counted and resuspended at 10x10^6 cells/ml.
  • 7. 100 ul of cells (1x10^6) were added to their respective tubes.
  • 8. Add 10ul of anti-CD20-APC (Sino Biological) to the appropriate tubes and incubate for 20 minutes on ice.
  • 9. Add 1ml PBS/BSA. Spin the cells down and resuspend in 0.5ml PBS/BSA
  • 10. Analyze
Validierungsbilder
Flow Cytometry validation image for anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody (APC) (ABIN2104927) Figure 1: Histogram of peripheral blood lymphocytes stained with anti-CD20-APC (red),...
Flow Cytometry validation image for anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody (APC) (ABIN2104927) Figure 2: Dot plot of peripheral blood lymphocytes stained with anti-CD20-APC (x-axis...
Immunogen Human CD20 Transfected CHO Cells
Klon 001
Isotyp IgG
Spezifität Human CD20 / MS4A1
Produktmerkmale This antibody was obtained from a rabbit immunized with purified, Human CD20 Transfected CHO Cells and conjugated with APC under optimum conditions, the unreacted APC was removed.
Andere Bezeichnung MS4A1 (MS4A1 Antibody Abstract)
Forschungsgebiet Immunology
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.1 mg/mL
Buffer Aqueous solution containing 0.5 % BSA and 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Lagerung 4 °C
Informationen zur Lagerung This antibody is stable for 12 months from date of receipt when stored at 2°C-8°C. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Haltbarkeit 12 months
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody (APC) (ABIN2214511) anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody (APC)
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