You are viewing an incomplete version of our website. Please click to reload the website as full version.

CD20 Antikörper (Membrane-Spanning 4-Domains, Subfamily A, Member 1) (APC)

Details für Produkt anti-MS4A1 Antikörper Nr. ABIN2214511, Anbieter: Anmelden zum Anzeigen
Antigen
  • AA960661
  • B1
  • Bp35
  • bp35
  • cd20
  • CD20
  • Cd20
  • cd20-like
  • CVID5
  • leu-16
  • LEU-16
  • Ly-44
  • MS4A1
  • MS4A2
  • ms4a2
  • Ms4a2
  • ms4a4c
  • S7
Reaktivität
Human
853
69
44
28
22
21
17
13
7
5
3
3
2
1
1
Wirt
Kaninchen
663
173
21
18
3
1
1
Klonalität
Monoklonal
Konjugat
Dieser CD20 Antikörper ist konjugiert mit APC
72
66
46
38
18
16
15
14
13
13
10
10
9
9
9
9
9
7
6
6
6
6
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS)
588
172
159
145
128
101
101
93
38
14
14
12
9
8
5
5
4
3
2
2
2
1
1
1
1
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen

Bilder

'Independent Validation' Siegel
Chargennummer HG08OC1306
Validierte Anwendung Flow Cytometry
Positivkontrolle peripheral blood mononuclear cells
Negativkontrolle peripheral blood mononuclear cells
Bewertung None
Primärantikörper
Kontrollen
  • Positive control: peripheral blood mononuclear cells stained with anti-CD20
  • Negative control: peripheral blood mononuclear cells that are CD20 negative and unstained cells
  • Isotype Control: None
  • Secondary Only control: None
Protokoll
  • 1. 10mls of blood (collected in EDTA) was diluted to 20mls with PBS + 0.5% BSA
  • 2. 10mls of diluted blood was overlaid onto 5mls of ficoll.
  • 3. Spin for 20 minutes at 1500 rpm with break off.
  • 4. The buffy coat layer was collected and wash with PBS/BSA.
  • 5. Residual red blood cells were lysed by resuspending the cells in 1ml ammonium chloride for 5-10 minutes at room temperature.
  • 6. The cells were washed with PBS/BSA, counted and resuspended at 10x10^6 cells/ml.
  • 7. 100 ul of cells (1x10^6) were added to their respective tubes.
  • 8. Add 10ul of anti-CD20-APC (Sino Biological) to the appropriate tubes and incubate for 20 minutes on ice.
  • 9. Add 1ml PBS/BSA. Spin the cells down and resuspend in 0.5ml PBS/BSA
  • 10. Analyze
Validierungsbilder
Flow Cytometry validation image for anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody (APC) (ABIN2104927) Figure 1: Histogram of peripheral blood lymphocytes stained with anti-CD20-APC (red),...
Flow Cytometry validation image for anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody (APC) (ABIN2104927) Figure 2: Dot plot of peripheral blood lymphocytes stained with anti-CD20-APC (x-axis...
Andere Bezeichnung MS4A1 (MS4A1 Antibody Abstract)
Forschungsgebiet Immunology
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Lagerung 4 °C
Informationen zur Lagerung This antibody is stable for 12 months from date of receipt when stored at 2 °C-8 °C.
Haltbarkeit 12 months
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD20 Antikörper (Membrane-Spanning 4-Domains, Subfamily A, Member 1)  (APC) (ABIN2214511) anti-Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1) antibody (APC)
Haben Sie etwas anderes gesucht?