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CD4 Antikörper (CD4 Molecule) (FITC)

Details für Produkt anti-CD4 Antikörper Nr. ABIN2213334, Anbieter: Anmelden zum Anzeigen
Antigen
  • cd4
  • CD4mut
  • L3T4
  • Ly-4
  • p55
  • W3/25
Alternativen
anti-Human CD4 Antikörper für Flow Cytometry
Reaktivität
Human
1163
655
241
72
56
43
26
23
22
21
19
15
15
15
11
10
9
8
7
7
6
5
5
5
3
2
2
2
1
1
1
Wirt
Maus
1331
624
273
8
4
1
Klonalität
Monoklonal
Konjugat
Dieser CD4 Antikörper ist konjugiert mit FITC
274
237
171
106
45
44
42
35
35
35
33
33
24
22
21
20
20
18
17
17
17
17
16
15
15
13
13
8
8
7
7
7
6
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS)
1744
424
402
336
304
301
181
139
117
68
37
28
28
27
22
18
14
14
11
9
7
6
6
6
5
4
3
2
2
2
2
2
2
2
1
1
1
1
1
1
Hersteller
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Hersteller Produkt- Nr.
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Bilder

'Independent Validation' Siegel
Chargennummer HG08FE1721
Validierte Anwendung Flow Cytometry
Positivkontrolle peripheral blood mononuclear cells
Negativkontrolle peripheral blood mononuclear cells
Bewertung This antibody performed well and is comparable to anti-CD4-FITC from BD Pharmingen (see data below)
Primärantikörper
Kontrollen
  • Positive control: peripheral blood mononuclear cells stained with anti-CD4
  • Negative control: peripheral blood mononuclear cells that are CD4 negative and unstained cells
  • Isotype Control: None
  • Secondary Only control: None
Protokoll
  • 1. 10mls of blood (collected in EDTA) was diluted to 20mls with PBS + 0.5% BSA
  • 2. 10mls of diluted blood was overlaid onto 5mls of ficoll.
  • 3. Spin for 20 minutes at 1500 rpm with break off.
  • 4. The buffy coat layer was collected and wash with PBS/BSA.
  • 5. Residual red blood cells were lysed by resuspending the cells in 1ml ammonium chloride for 5-10 minutes at room temperature.
  • 6. The cells were washed with PBS/BSA, counted and resuspended at 10x10^6 cells/ml.
  • 7. 100ul of cells (1x10^6) were added to their respective tubes.
  • 8. Add 5µl of anti-CD4-FITC (Sino Biological) or 20µl of anti-CD4-FITC (BD Pharmingen) to the appropriate tubes and incubate for 20 minutes on ice.
  • 9. Add 1ml PBS/BSA. Spin the cells down and resuspend in 0.5ml PBS/BSA.
  • 10. Analyze
Validierungsbilder
Flow Cytometry validation image for anti-CD4 Molecule (CD4) antibody (FITC) (ABIN2104933) Figure 1: Histogram of peripheral blood lymphocytes stained with anti-CD4-FITC (red),...
Flow Cytometry validation image for anti-CD4 Molecule (CD4) antibody (FITC) (ABIN2104933) Figure 2: Histogram of peripheral blood lymphocytes stained with anti-CD4-FITC from B...
Flow Cytometry validation image for anti-CD4 Molecule (CD4) antibody (FITC) (ABIN2104933) Figure 3: Dot plot of peripheral blood lymphocytes stained with anti-CD4-FITC (x-axis...
Andere Bezeichnung CD4 (CD4 Antibody Abstract)
Forschungsgebiet Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Handhabung Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Lagerung 4 °C
Informationen zur Lagerung This antibody is stable for 12 months from date of receipt when stored at 2 °C-8 °C.
Haltbarkeit 12 months
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD4 Antikörper (CD4 Molecule)  (FITC) (ABIN2213334) anti-CD4 Molecule (CD4) antibody (FITC)