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CD4 Antikörper (CD4 Molecule) (AA 1-390) (FITC)

Details für Produkt anti-CD4 Antikörper Nr. ABIN2213334, Anbieter: Anmelden zum Anzeigen
Antigen
  • cd4
  • L3T4
  • Ly-4
  • CD4mut
  • W3/25
  • p55
  • CD4 molecule
  • CD4 antigen
  • T-cell surface glycoprotein CD4
  • Cd4 molecule
  • CD4
  • cd4
  • Cd4
Alternativen
anti-Human CD4 Antikörper für Flow Cytometry
Epitop
AA 1-390
66
62
21
17
15
15
15
12
12
10
9
8
8
8
8
6
6
5
5
5
5
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human
1161
610
233
68
66
40
34
22
22
21
19
18
16
15
15
12
10
10
9
9
6
5
5
5
4
2
2
2
1
1
1
1
Wirt
Maus
1313
568
271
10
4
1
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser CD4 Antikörper ist konjugiert mit FITC
257
221
163
100
47
47
45
40
36
34
33
29
27
23
21
21
19
19
18
18
18
18
15
15
14
13
13
13
11
8
7
6
6
5
5
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS)
1661
463
428
328
306
283
150
143
85
61
39
38
29
29
22
19
18
14
14
11
9
7
6
6
5
4
4
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Bilder

'Independent Validation' Siegel
Chargennummer HG08FE1721
Validierte Anwendung Flow Cytometry
Positivkontrolle peripheral blood mononuclear cells
Negativkontrolle peripheral blood mononuclear cells
Bewertung This antibody performed well and is comparable to anti-CD4-FITC from BD Pharmingen (see data below)
Primärantikörper
Kontrollen
  • Positive control: peripheral blood mononuclear cells stained with anti-CD4
  • Negative control: peripheral blood mononuclear cells that are CD4 negative and unstained cells
  • Isotype Control: None
  • Secondary Only control: None
Protokoll
  • 1. 10mls of blood (collected in EDTA) was diluted to 20mls with PBS + 0.5% BSA
  • 2. 10mls of diluted blood was overlaid onto 5mls of ficoll.
  • 3. Spin for 20 minutes at 1500 rpm with break off.
  • 4. The buffy coat layer was collected and wash with PBS/BSA.
  • 5. Residual red blood cells were lysed by resuspending the cells in 1ml ammonium chloride for 5-10 minutes at room temperature.
  • 6. The cells were washed with PBS/BSA, counted and resuspended at 10x10^6 cells/ml.
  • 7. 100ul of cells (1x10^6) were added to their respective tubes.
  • 8. Add 5µl of anti-CD4-FITC (Sino Biological) or 20µl of anti-CD4-FITC (BD Pharmingen) to the appropriate tubes and incubate for 20 minutes on ice.
  • 9. Add 1ml PBS/BSA. Spin the cells down and resuspend in 0.5ml PBS/BSA.
  • 10. Analyze
Validierungsbilder
Flow Cytometry validation image for anti-CD4 Molecule (CD4) (AA 1-390) antibody (FITC) (ABIN2104933) Figure 1: Histogram of peripheral blood lymphocytes stained with anti-CD4-FITC (red),...
Flow Cytometry validation image for anti-CD4 Molecule (CD4) (AA 1-390) antibody (FITC) (ABIN2104933) Figure 2: Histogram of peripheral blood lymphocytes stained with anti-CD4-FITC from B...
Flow Cytometry validation image for anti-CD4 Molecule (CD4) (AA 1-390) antibody (FITC) (ABIN2104933) Figure 3: Dot plot of peripheral blood lymphocytes stained with anti-CD4-FITC (x-axis...
Immunogen Recombinant Human CD4 protein (ABIN2002782)
Klon 08
Isotyp IgG1
Spezifität Human CD4
Produktmerkmale This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD4 (rh CD4, ABIN2002782 NP_000607.1, Met1-Trp390, and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
Andere Bezeichnung CD4 (CD4 Antibody Abstract)
Forschungsgebiet Immunology, Adaptive Immunity, Hematopoietic Progenitors, CD Antigens, Surface Receptors of Immune Cells
Pathways TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.1 mg/mL
Buffer Aqueous solution containing 0.5 % BSA and 0.09 % sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Protected from prolonged exposure to light. Do not freeze ! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal
Lagerung 4 °C
Informationen zur Lagerung This antibody is stable for 12 months from date of receipt when stored at 2°C-8°C. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Haltbarkeit 12 months
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-CD4 Antikörper (CD4 Molecule) (AA 1-390) (FITC) (ABIN2213334) anti-CD4 Molecule (CD4) (AA 1-390) antibody (FITC)