Esel anti-Ziege IgG (Heavy & Light Chain) Antikörper (IRDye800CW)

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Antigen
Epitop
Heavy & Light Chain
4680
2537
1879
1066
1006
860
298
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reaktivität
Ziege
2850
2735
2296
1675
1432
788
613
442
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Wirt
Esel
6705
4831
1794
891
609
388
91
55
52
21
8
5
5
5
2
1
1
Klonalität
Polyklonal
Konjugat
IRDye800CW
2236
1872
1738
1356
767
682
519
297
264
223
222
200
156
138
128
110
79
75
69
55
51
46
43
40
38
36
34
34
32
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
15
15
15
15
15
15
14
12
12
12
10
10
9
9
9
9
9
9
8
8
8
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
In-Gel Western blotting (gelWB), Immunohistochemistry (IHC), Western Blotting (WB)
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Hersteller
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Hersteller Produkt- Nr.
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Marke In-Cell Western™,CellTag™
Immunogen Goat IgG, whole molecule
Isotyp IgG
Fragment H&L
Spezifität Goat IgG
Kreuzreaktivität Schaf
Kreuzreaktivität (Details) The antibody also reacts with sheep IgG.
Produktmerkmale IRDye 800CW Donkey anti-Goat IgG is a highly cross-adsorbed secondary antibody suitable for use in a variety of applications including Western blotting, In-Cell Western Assays, In-Gel Westerns, and many others. Based on immunoelectrophoresis, the antibody reacts with the heavy chains on goat IgG and with the light chains common to most goat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody has been tested by ELISA and/or solid-phase adsorbed to ensure minimal cross-reaction with chicken, guinea pig, Syrian hamster, horse, human, mouse, rabbit, and rat serum proteins, but the antibody may cross-react with immunoglobulins from other species.
Blocking buffers and antibody diluents made with bovine serum albumin (BSA) and dry milk may contain IgG that reacts with anti-bovine IgG, anti-goat IgG, anti-horse IgG, and anti-sheep IgG antibodies. This can lead to a significant increase in background and/or reduction of secondary antibody titer for protein detection applications.
Reinigung immunoaffinity chromatography
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise Western blot: 1:5,000 - 1:25,000
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Rekonstitution Reconstitute with sterile, distilled water
Konzentration 1.0 mg/mL
Buffer PBS, pH 7.4
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Protect from light.
Lagerung 4 °C
Produkt verwendet in: Li, Li, Sun, Dong, Li, Zhao, Guo, Weng, Qiu: "Poly(C)-binding protein 1, a novel N(pro)-interacting protein involved in classical swine fever virus growth." in: Journal of virology, Vol. 87, Issue 4, pp. 2072-80, 2013 (PubMed).