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ZBTB33 Antikörper (AA 103-584)

ZBTB33 Reaktivität: Human, Maus, Ratte, Hund, Huhn, Hamster WB, IP, IHC Wirt: Maus Monoclonal unconjugated
Produktnummer ABIN210906
  • Target Alle ZBTB33 Antikörper anzeigen
    ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))
    Bindungsspezifität
    • 5
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    AA 103-584
    Reaktivität
    • 26
    • 14
    • 11
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte, Hund, Huhn, Hamster
    Wirt
    • 24
    • 3
    Maus
    Klonalität
    • 23
    • 4
    Monoklonal
    Konjugat
    • 16
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dieser ZBTB33 Antikörper ist unkonjugiert
    Applikation
    • 12
    • 12
    • 6
    • 5
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
    Spezifität
    Recognizes Kaiso, Mr 97kD. Species cross-reactivity: Human, mouse, rat, canine, chicken, hamster and simian.
    Aufreinigung
    Protein G purified
    Immunogen
    Recombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584).

    Type of Immunogen: Recombinant protein
    Isotyp
    IgG1
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  • Applikationshinweise
    Approved: IHC (1 μg/mL), IP, WB (0.5 - 2 μg/mL)

    Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Immunohistochemistry: 1 μg/mL gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100 % methanol. Western Blot: 0.5-2 μg/mL detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 μg immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3. Supershift: Supershifts a specific methyl-CpG complex.
    Kommentare

    Target Species of Antibody: Mouse

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    Lot specific
    Buffer
    0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Avoid repeat freeze-thaw cycles.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target
    ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))
    Andere Bezeichnung
    ZBTB33 / Kaiso (ZBTB33 Produkte)
    Synonyme
    kaiso antikoerper, ZNF-kaiso antikoerper, ZNF348 antikoerper, AW260253 antikoerper, E130014G12Rik antikoerper, Kaiso antikoerper, Znf-kaiso antikoerper, RGD1566309 antikoerper, zinc finger and BTB domain containing 33 S homeolog antikoerper, zinc finger and BTB domain containing 33 antikoerper, zbtb33.S antikoerper, ZBTB33 antikoerper, Zbtb33 antikoerper
    Hintergrund
    Name/Gene ID: ZBTB33

    Synonyms: ZBTB33, Kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, Kaiso transcription factor, ZNF348
    Gen-ID
    10009
    UniProt
    Q86T24
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