ZBTB33 Antikörper (AA 103-584)
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- Target Alle ZBTB33 Antikörper anzeigen
- ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))
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Bindungsspezifität
- AA 103-584
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Reaktivität
- Human, Maus, Ratte, Hund, Huhn, Hamster
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser ZBTB33 Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
- Spezifität
- Recognizes Kaiso, Mr 97kD. Species cross-reactivity: Human, mouse, rat, canine, chicken, hamster and simian.
- Aufreinigung
- Protein G purified
- Immunogen
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Recombinant N-terminal GST and His-tagged fusion protein corresponding to the central region of murine Kaiso (aa 103-584).
Type of Immunogen: Recombinant protein - Isotyp
- IgG1
- Top Product
- Discover our top product ZBTB33 Primärantikörper
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- Applikationshinweise
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Approved: IHC (1 μg/mL), IP, WB (0.5 - 2 μg/mL)
Usage: Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation and Supershift. Immunohistochemistry: 1 μg/mL gives positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100 % methanol. Western Blot: 0.5-2 μg/mL detects Kaiso in RIPA lysates from 3T3/A31 cells. 3T3/A31 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Kaiso (2 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 2-5 μg immunoprecipitates Kaiso from RIPA lysates from NIH 3T3 cells.1, 3. Supershift: Supershifts a specific methyl-CpG complex. - Kommentare
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Target Species of Antibody: Mouse
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- Lot specific
- Buffer
- 0.1 M Tris-glycine, pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeat freeze-thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- ZBTB33 (Zinc Finger and BTB Domain Containing 33 (ZBTB33))
- Andere Bezeichnung
- ZBTB33 / Kaiso (ZBTB33 Produkte)
- Synonyme
- kaiso antikoerper, ZNF-kaiso antikoerper, ZNF348 antikoerper, AW260253 antikoerper, E130014G12Rik antikoerper, Kaiso antikoerper, Znf-kaiso antikoerper, RGD1566309 antikoerper, zinc finger and BTB domain containing 33 S homeolog antikoerper, zinc finger and BTB domain containing 33 antikoerper, zbtb33.S antikoerper, ZBTB33 antikoerper, Zbtb33 antikoerper
- Hintergrund
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Name/Gene ID: ZBTB33
Synonyms: ZBTB33, Kaiso, WUGSC:H_DJ525N14.1, ZNF-kaiso, Kaiso transcription factor, ZNF348 - Gen-ID
- 10009
- UniProt
- Q86T24
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