CHO Cells anti-Human IgG1 (AA 99-330), (Cys103Ser-Mutant) Antikörper

Details zu Produkt Nr. ABIN2001825, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
AA 99-330, Cys103Ser-Mutant
110
54
12
11
9
8
7
5
5
4
4
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human
268
145
94
35
22
9
6
3
3
2
2
1
1
1
1
1
Wirt
CHO Cells
183
173
117
69
33
5
3
3
1
Antikörpertyp
Recombinant
Applikation
SDS-PAGE (SDS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Fragment Fc fragment
Produktmerkmale The recombinant human IgG1 Fc consists of 232 amino acids and has a predicted molecular mass of 26 kDa. As a result of glycosylation, the apparent molecular mass of rhFc is approximately 34 kDa in SDS-PAGE under reducing conditions.

Protein Structure: A DNA sequence encoding the human IgG1 Fc region (AAC82527.1) (Glu 99-Lys 330) (one aa mutation,103 Cys/Ser) was expressed.
Predicted N-Term: Glu 99
Reinheit > 92 % as determined by SDS-PAGE
Endotoxin-Niveau < 1.0 EU per μg of the protein as determined by the LAL method
Hintergrund Synonyms: IgG1 Fc,Ighg1
Molekulargewicht 26 kDa, 34 kDa
Forschungsgebiet Secondary Antibodies
Applikationshinweise Optimal working dilution should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer Lyophilized from sterile PBS, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.
Handhabung Avoid repeated freeze-thaw cycles. It is recommended that the protein be aliquoted for optimal storage.
Lagerung -20 °C,-80 °C
Informationen zur Lagerung Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Haltbarkeit 12 months
Bilder des Herstellers
SDS-PAGE (SDS) image for CHO Cells anti-Human IgG1 (AA 99-330), (Cys103Ser-Mutant) antibody (ABIN2001825) CHO Cells anti-Human IgG1 (AA 99-330), (Cys103Ser-Mutant) antibody
Allgemeine Veröffentlichungen Sprague, Martin, Bjorkman: "pH dependence and stoichiometry of binding to the Fc region of IgG by the herpes simplex virus Fc receptor gE-gI." in: The Journal of biological chemistry, Vol. 279, Issue 14, pp. 14184-93, 2004

Sprague, Martin, Bjorkman: "pH dependence and stoichiometry of binding to the Fc region of IgG by the herpes simplex virus Fc receptor gE-gI." in: The Journal of biological chemistry, Vol. 279, Issue 14, pp. 14184-93, 2004 (PubMed).

Gómez-Guerrero, Duque, Casado, Pastor, Blanco, Mampaso, Vivanco, Egido: "Administration of IgG Fc fragments prevents glomerular injury in experimental immune complex nephritis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 2092-101, 2000 (PubMed).

Pfefferkorn, van de Winkel, Swink: "A novel role for IgG-Fc. Transductional potentiation for human high affinity Fc gamma receptor (Fc gamma RI) signaling." in: The Journal of biological chemistry, Vol. 270, Issue 14, pp. 8164-71, 1995 (PubMed).

Eckle, Kolb, Heiser, Havemann: "Stimulation of neutrophil elastase and myeloperoxidase release by IgG fragments." in: Clinical and experimental immunology, Vol. 81, Issue 2, pp. 352-6, 1990 (PubMed).

Carosella, Gay, Armand, Touraine: "Human B-cell differentiation by Fc fragment of IgG. I. Fc fragment from human IgG induces plasma cell generation but cannot induce lymphocyte proliferation." in: Cellular immunology, Vol. 112, Issue 2, pp. 262-70, 1988 (PubMed).