Tumor Necrosis Factor Antikörper (TNF) (AA 77-233)

Details for Product anti-TNF Antibody No. ABIN1997163, Anbieter: Anmelden zum Anzeigen
Antigen
  • TNF
  • tnf
  • dif
  • tnfa
  • xtnf
  • TNF-a
  • tnfsf2
  • tnf-alpha
  • TNF-alpha
  • DIF
  • TNFA
  • TNFSF2
  • TNFalpha
  • Tnfa
  • Tnfsf1a
  • RATTNF
  • TNFa
  • cTNF
  • TNF-ALPHA
  • Cachectin
  • Tnf-alpha
  • tnfa-like
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor
  • tumour necrosis factor alpha-like
  • tumor necrosis factor alpha
  • lipopolysaccharide-induced TNF factor
  • tumor necrosis factor b (TNF superfamily, member 2)
  • TNF
  • tnf
  • tnf-alpha
  • LITAF
  • Tnf
  • tnfb
Alternativen
anti-Human Tumor Necrosis Factor Antikörper für Activity Inhibition
Epitop
AA 77-233
51
43
29
27
24
22
17
17
15
11
11
10
10
8
4
4
4
3
3
3
3
2
2
1
1
1
1
1
1
1
1
1
Reaktivität
Human
1254
460
358
145
136
119
119
63
55
41
29
18
14
12
9
7
6
4
4
4
4
2
1
1
1
1
Wirt
Maus
939
516
184
48
10
9
7
6
4
3
2
Klonalität (Klon)
Monoklonal ()
Konjugat
Dieser Tumor Necrosis Factor Antikörper ist unkonjugiert
169
100
74
50
32
30
28
27
26
25
25
25
25
25
24
24
24
23
13
9
4
4
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Cell Culture (CC), ELISA, Flow Cytometry (FACS)
886
859
621
322
286
230
219
190
137
118
98
74
29
27
26
22
20
18
14
9
7
7
6
6
6
5
5
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Recombinant Human TNFalpha protein (ABIN2003122)
Klon 9A7A5G9
Isotyp IgG1
Spezifität Human TNFα
No cross-reactivity in WB and ELISA with
E.coli cell lysate
Keine Kreuzreaktivität Escherichia coli (E. coli)
Produktmerkmale This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Tumor necrosis factor-alpha (rhTNFα, ABIN2003122 Val 77-Leu 233, NP_000585.2,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Reinigung purified by Protein A affinity chromatography
Sterilität 0.2 μm filtered
Andere Bezeichnung TNF (TNF Antibody Abstract)
Hintergrund Tumor necrosis factor alpha (TNFα), also known as cachectin and TNFSF1A, is the prototypic cytokine of the the TNF superfamily. It is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNFα is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). Two receptors mediating the TNFα function have been identified, the ubiquitous TNF RI from which most signaling are derived, and the hematopoietic cell-restricted TNFRII. TNFα has been implicated in the pathogenesis of a variety of human diseases including autoimmune diseases, insulin resistance, and cancer.
NCBI Accession NP_000585.2
Pathways NF-kappaB Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus
Applikationshinweise ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human TNFα. The detection limit for Human TNFα is approximately 0.6 ng/well.
ELISA(Cap): 0.5-4 μg/mL
FCM: 0.5-2 μg/Test
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Buffer 0.2 μm filtered solution in PBS
Konservierungsmittel Without preservative
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung 4 °C,-20 °C,-80 °C
Informationen zur Lagerung This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Haltbarkeit 12 months
Bilder des Herstellers
Flow Cytometry (FACS) image for anti-Tumor Necrosis Factor Antikörper (TNF) (AA 77-233) (ABIN1997163) Flow cytometric analysis of Purified anti-Human TNFa on CHO transfected cells and sti...
Produkt verwendet in: Cohen, Sabhachandani, Golberg, Konry: "Approaching near real-time biosensing: microfluidic microsphere based biosensor for real-time analyte detection." in: Biosensors & bioelectronics, Vol. 66, pp. 454-60, 2014 (PubMed).

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