Progesterone Receptor (PGR) Antikörper

Antigen: Progesterone Receptor (PGR)

Synonyme: PR, NR3C3, PR-A, PR-B, BB114106, 9930019P03Rik, ENSMUSG00000074510, PGR, pgr-A, XPR-1, LOC443444, pg, pr, gb:dq017620

Klonalität: Monoklonal (BV7)

Wirt: Kaninchen

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN197732

Produktbeschreibung

Weitere Bezeichnung: Progesterone Receptor

Immunogen: A synthetic cyclic peptides from the antigen

Format: Liquid (prediluted)

Isotyp: IgG  (Passende Sekundärantikörper)

Klon: BV7

Spezifität: The progesterone receptor is an intracellular steroid receptor that specifically binds progesterone. Expressed by a single gene (chromosome 11q22), it has two main forms, A and B, that differ in their molecular weight. It has been proposed that expression of PR determination indicates a responsive estrogen receptor (ER) pathway, and therefore, may predict likely response to endocrine therapy in human breast cancer. A number of studies have shown that PR determination provides supplementary information to ER, in predicting response to endocrine therapy as well as estimating survival. PR has proved superior to ER as a prognostic indicator in some studies.

Anwendungen

Applikationshinweise: Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Nuclear

Konzentration: 1 to 4 ug/ml

Buffer: This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Feil, Clarke, Satyaswaroop: "Progestin-mediated changes in progesterone receptor forms in the normal human endometrium." in: Endocrinology, Vol. 123, Issue 5, pp. 2506-13, 1988 (PubMed).

Clarke, Zaino, Feil et al.: "Monoclonal antibodies to human progesterone receptor: characterization by biochemical and immunohistochemical techniques." in: Endocrinology, Vol. 121, Issue 3, pp. 1123-32, 1987 (PubMed).

Tesch, Shawwa, Henderson: "Immunohistochemical determination of estrogen and progesterone receptor status in breast cancer." in: American journal of clinical pathology, Vol. 99, Issue 1, pp. 8-12, 1993 (PubMed).