CD25 Antikörper
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| Klonalität |
Monoklonal (IL2R-1) |
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Wirt
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Reaktivität
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Applikation
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Durchflusszytometrie (FACS) (200), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (18), Immunpräzipitation (IP) (16), Immunfluoreszenz (IF) (15), Blockierende Antikörper (Inhibition) (9), Immunhistochemie (IHC) (6), Funktionale Studien (Func) (3), Abreicherung (Depletion) (2), Western Blot (WB) (2), Blocking Antibody (Inhibition) (1), ELISA (1)
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| Produktnummer |
ABIN197714 |
| Menge |
6 ml (1 to 4 ug/ml) (Varianten) |
| Preis |
162,50 € Zzgl. Versandkosten €40,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 5 Werktagen |
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Weitere Bezeichnung
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CD25/IL-2R alpha
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Immunogen
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BALB/C mice were injected with recombinant human interleukin- 2 receptor.
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Format
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Liquid (prediluted)
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Klon
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IL2R-1
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Spezifität
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This antibody is specific to a 55 kD protein known as interleukin 2-receptor (IL-2R alpha). IL-2R alpha is over expressed on the surface of activated T cell, HTLV-1 or HTLV-2 positive T cell lines and moderately expressed on activated B cells, NK-cells and macrophages.
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Applikationshinweise
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Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Cell membrane
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Konzentration
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1 to 4 ug/ml
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Buffer
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This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required.
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Lagerung
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Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.
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Beschränkungen
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Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.
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Alternativen zu Antigen "CD25", Typ "Antikörper" finden
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Wirte
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Ratte (117), Maus (87)
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Reaktivitäten
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Maus (116), Human (65), Ratte (Rattus) (19), Affe (8), Rind (Kuh) (3), Cynomolgus (3), Rhesusaffen (3), Schwein (1)
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Applikationen
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Durchflusszytometrie (FACS) (200), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (18), Immunpräzipitation (IP) (16), Immunfluoreszenz (IF) (15), Blockierende Antikörper (Inhibition) (9), Immunhistochemie (IHC) (6), Funktionale Studien (Func) (3), Abreicherung (Depletion) (2), Western Blot (WB) (2), Blocking Antibody (Inhibition) (1), ELISA (1)
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Konjugate
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Biotin (27), PE (23), FITC (22), APC (14), Alexa Fluor 488 (13), PE,Cy7 (12), PerCP,Cy5.5 (7), Alexa Fluor 647 (6), Alexa Fluor 700 (6), Pacific Blue (6), PE,Cy5 (5), APC,Cy7 (4), RPE (4), SPRD (4), APC-eFluor 780 (2), PerCP (2), PE-Dyomics 647 (1), RPE,Cy5 (1)
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Alternativen 
