Epidermal Growth Factor Receptor (EGFR) Antikörper

Antigen: Epidermal Growth Factor Receptor (EGFR)

Synonyme: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Klonalität: Monoklonal (31G7)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN197682

Produktbeschreibung

Immunogen: Human EGFR derived from A-431 cell line.

Format: Concentrated

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 31G7

Spezifität: The epidermal growth factor receptor (EGFR) is a 170 kD transmembrane protein consisting of an extracellular EGF-binding domain, a short transmembrane region and an intracellular domain with ligand-activated tyrosine kinase activity. EGFR is expressed in many normal epithelial tissues, particularly in the basal layers of stratified epithelium and in squamous epithelium. Over expression of EGFR has been observed in many types of neoplasia, as a result of gene amplification and/or increased protein transcription.

Synonyme: ERBB, HER1, mENA, ERBB1, PIG61, Wa5, wa2, Erbb, Errp, wa-2, AI552599, 9030024J15Rik, ErbB-1, C-erb, D-EGFR, D-Egf, DEGFR, DER, DER flb, DER/EGFR, DER/faint little ball, DER/top, DER/torpedo, DmHD-33, EFG-R, EGF-R, EGFR, EK2-6, Egf, El, Elp, Elp-1, Elp-B1, Elp-B1RB1, HD-33, Torpedo/DER, Torpedo/Egfr, c-erbB, d-egf-r, dEGFR1, flb, l(2)05351, l(2)09261, l(2)57DEFa, l(2)57EFa, l(2)57Ea, pnt, top, top/DER, top/flb, DmelCG10079, CG10079, egfr, EGFR12, EGFR15, egfr1, Erbb2

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the use. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Breast carcinoma. Cellular localization: Membrane, cytoplasm

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as diluted ascites containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Rusch, Klimstra, Venkatraman: "Molecular markers help characterize neuroendocrine lung tumors." in: The Annals of thoracic surgery, Vol. 62, Issue 3, pp. 798-809; discussion 809-10, 1996 (PubMed).

Iwakura, Miwa, Sakai et al.: "Human hypertrophic nonunion tissue contains mesenchymal progenitor cells with multilineage capacity in vitro." in: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 27, Issue 2, pp. 208-15, 2009 (PubMed).