CD163 Molecule (CD163) Antikörper

Antigen: CD163 Molecule (CD163)

Synonyme: CD163, DMBT1, M130, MM130, CD163v2, CD163v3

Klonalität: Monoklonal (10D6)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (IHC)

Produktnummer: ABIN197678

Produktbeschreibung

Weitere Bezeichnung: CD163

Immunogen: Human CD163 antigen.

Format: Concentrated

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 10D6

Spezifität: The CD163 molecule is a type I membrane protein also known as M130 antigen, Ber-Mac3, Ki-M8 or SM4. CD163 protein is restricted in its expression to the monocytic/macrophage lineage. It is reported to be present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhans cells. In addition, multinucleated cells within inflammatory lesions are reported not to express CD163 protein. The protein is upregulated by glucocorticoids and downregulated by the immunosuppressant cyclosporin A and by phorbol esters.

Anwendungen

Applikationshinweise: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the use. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Tonsil. Cellular localization: Membrane

Konzentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant containing sodium azide as preservative.

Lagerung: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Beschränkungen: Für Forschungszwecke. Als CE-zertifizierter Antikörper in der Europäischen Union für In Vitro Diagnostik (IVD) zugelassen.

Publikationen

Publikationen: Hoegger, Dreier, Droste et al.: "Identification of the integral membrane protein RM3/1 on human monocytes as a glucocorticoid-inducible member of the scavenger receptor cysteine-rich family (CD163)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 161, Issue 4, pp. 1883-90, 1998 (PubMed).

Droste, Sorg, Hoegger: "Shedding of CD163, a novel regulatory mechanism for a member of the scavenger receptor cysteine-rich family." in: Biochemical and biophysical research communications, Vol. 256, Issue 1, pp. 110-3, 1999 (PubMed).