MAPKAP Kinase 2 Antikörper (pThr334)

Produktnummer ABIN197078

Produktdetails anti-MAPKAP Kinase 2 Antikörper

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Bindungsspezifität: pThr334

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MAPKAP Kinase 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Spezifität: This antibody detects endogenous levels of MAPKAPK-2 only when phosphorylated at Threonine 334.

Aufreinigung: Affinity Chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human MAPKAPK-2 around the phosphorylation site of threonine 334 (P-Q-TP-P-L).

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500approx. 1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody (in aliquots) at -20 °C.

Details zu MAPKAP Kinase 2

Target: MAPKAP Kinase 2 (MAPKAPK2) (Mitogen-Activated Protein Kinase-Activated Protein Kinase 2 (MAPKAPK2))

Andere Bezeichnung: MAPKAP Kinase-2 (MAPKAPK2 Produkte)

Synonyme: mapkapk2 antikoerper, wu:fb30e10 antikoerper, wu:fc56c10 antikoerper, wu:fi03a01 antikoerper, MGC78852 antikoerper, MAPKAPK2 antikoerper, AA960234 antikoerper, MAPKAP-K2 antikoerper, MK-2 antikoerper, MK2 antikoerper, Rps6kc1 antikoerper, mitogen-activated protein kinase-activated protein kinase 2a antikoerper, mitogen-activated protein kinase-activated protein kinase 2 L homeolog antikoerper, mitogen-activated protein kinase-activated protein kinase 2 antikoerper, MAP kinase-activated protein kinase 2 antikoerper, mapkapk2a antikoerper, mapkapk2.L antikoerper, MAPKAPK2 antikoerper, Mapkapk2 antikoerper

Hintergrund: MAP kinase activated protein kinase 2 (MAPKAP Kinase 2), also known as p45 hsp27 kinase, is a 45-54 kDa serine/threonine protein kinase that contains a proline rich sequence and two putative SH3 binding sites. MAPKAP Kinase 2 is activated in response to stress, IL1 and TNF, possibly catalyzed by p38/Hog dependent phosphorylation. One of the major substrates of MAPKAP Kinase 2 is hsp27, which stimulates actin polymerization in order to facilitate recovery from destruction of cytoskeleton during cellular stresses. MAPKAP2 is implicated in several disorders including ischemic brain injury and heart failure and has been shown to be important in regulating stress resistance and the production of TNF alpha.Synonyms: MAP kinase-activated protein kinase 2, MAPK-activated protein kinase 2, MAPKAP kinase 2, MAPKAPK-2, MAPKAPK2, MK2

Gen-ID: 9261

NCBI Accession: NP_004750

UniProt: P49137

Pathways: MAPK Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Toll-Like Receptors Cascades