MTOR Antikörper (Mechanistic Target of Rapamycin (serine/threonine Kinase)) (pSer2448)

Details for Product anti-FRAP1 Antibody No. ABIN196962, Anbieter: Anmelden zum Anzeigen
Antigen
  • FRAP1
  • FRAP
  • FRAP2
  • RAFT1
  • RAPT1
  • 2610315D21Rik
  • AI327068
  • Frap1
  • flat
  • frap1
  • tor
  • wu:fc22h08
  • mechanistic target of rapamycin (serine/threonine kinase)
  • MTOR
  • Mtor
  • mtor
Epitop
pSer2448
65
36
28
23
21
15
15
13
12
10
8
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Reaktivität
Human, Maus, Ratte (Rattus)
378
200
197
18
9
8
7
7
7
7
4
2
2
2
1
1
Wirt
Kaninchen
324
49
4
1
Klonalität
Polyklonal
Konjugat
Dieser MTOR Antikörper ist unkonjugiert
8
8
7
5
5
4
4
4
4
4
4
4
4
4
4
4
3
3
Applikation
Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
252
114
99
74
56
52
37
22
8
7
7
7
3
2
2
2
2
1
1
Optionen
Hersteller
Anmelden zum Anzeigen
Hersteller Produkt- Nr.
Anmelden zum Anzeigen
Immunogen Synthesized phosphopeptide derived from human mTOR around the phosphorylation site of serine 2448 (T-D-SP-Y-S)
Spezifität This antibody detects endogenous levels of mTOR only when phosphorylated at serine 2448.
Reinigung Affinity Chromatography using epitope-specific phosphopeptide.
Andere Bezeichnung mTOR / FRAP1 (FRAP1 Antibody Abstract)
Hintergrund MTOR, or FKBP12 rapamycin associated protein (FRAP), is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 289- kDa protein (symbolized RAFT1) with significant homology to the Saccharomyces cerevisiae protein TOR1 and has been shown to associate with the immunophilin FKBP12 in a rapamycin dependent fashion. The FKBP12-rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast. The binding of FRAP to FKBP12-rapamycin correlated with the ability of these ligands to inhibit cell cycle progression.Synonyms: FK506-binding protein 12-rapamycin complex-associated protein 1, FKBP12-rapamycin complex-associated protein, FRAP, FRAP2, Mammalian target of rapamycin, RAPT1, Rapamycin target protein, Serine/threonine-protein kinase mTOR, TOR
Molekulargewicht 289 kDa (Predicted)
Gen-ID 2475
NCBI Accession NP_004949
UniProt P42345
Pathways PI3K-Akt Signalweg, RTK Signalweg, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagie, CXCR4-mediated Signaling Events
Applikationshinweise Immunofluorescence: 1/100-1/200. Western Blot: 1/500-1/1000. Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Konzentration 1.0 mg/mL
Buffer PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung -20 °C
Informationen zur Lagerung Store the antibody (in aliquots) at -20 °C.
Bilder des Herstellers
 image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN196962) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody
 image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN196962) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (Image 2)
 image for anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (ABIN196962) anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (FRAP1) (pSer2448) antibody (Image 3)
Allgemeine Veröffentlichungen Holz, Blenis: "Identification of S6 kinase 1 as a novel mammalian target of rapamycin (mTOR)-phosphorylating kinase." in: The Journal of biological chemistry, Vol. 280, Issue 28, pp. 26089-93, 2005 (PubMed).

Chiang, Abraham: "Phosphorylation of mammalian target of rapamycin (mTOR) at Ser-2448 is mediated by p70S6 kinase." in: The Journal of biological chemistry, Vol. 280, Issue 27, pp. 25485-90, 2005 (PubMed).

Mothe-Satney, Gautier, Hinault, Lawrence, Van Obberghen: "In rat hepatocytes glucagon increases mammalian target of rapamycin phosphorylation on serine 2448 but antagonizes the phosphorylation of its downstream targets induced by insulin and amino acids." in: The Journal of biological chemistry, Vol. 279, Issue 41, pp. 42628-37, 2004 (PubMed).

Bolster, Kubica, Crozier, Williamson, Farrell, Kimball, Jefferson: "Immediate response of mammalian target of rapamycin (mTOR)-mediated signalling following acute resistance exercise in rat skeletal muscle." in: The Journal of physiology, Vol. 553, Issue Pt 1, pp. 213-20, 2003 (PubMed).

Haben Sie etwas anderes gesucht?