The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. Vav (phospho-Tyr174) antibody detects endogenous levels of Vav only when phosphorylated at tyrosine 174.
Aufreinigung
Immunoaffinity chromatography.
Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human Vav around the phosphorylation site of tyrosine 174 (E-I-YP-E-D).
VAV1
Reaktivität: Human
WB, IHC, ICC
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
Suitable for use in Western blot (1: 500approx. 1: 1000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1.0 mg/mL
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.