HIS Tag Antikörper (HRP)
| Antigen |
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| Klonalität |
Polyklonal |
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Wirt
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Konjugat
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HRP (11), Biotin (10), FITC (8), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alkaline Phosphatase (AP) (2), HiLyte Fluor 488 (2), HiLyte Fluor 555 (2), HiLyte Fluor 647 (2), PE,Cy5.5 (2), AMCA (1), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FAM (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1), ROX (1), Sepharose (1), TAMRA (1)
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Applikation
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 Alternativen Western Blot (WB), ELISA, Durchflusszytometrie (FACS)
Western Blot (WB) (117), ELISA (74), Immunpräzipitation (IP) (50), Immunfluoreszenz (IF) (45), Durchflusszytometrie (FACS) (30), Immunzytochemie (ICC) (13), Immunhistochemie (IHC) (10), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (6), Dot Blot (Dot) (5), Immunhistochemie (Paraffinschnitte) (IHC (p)) (5), Transfizierte Zellkultur (TCC) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1)
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| Produktnummer |
ABIN195464 |
| Menge |
500ug (Varianten) |
| Preis |
720,00 € Zzgl. Versandkosten €40,00, €20,00 Trockeneispauschale sowie MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
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Weitere Bezeichnung
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HIS-tag
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Immunogen
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Cys – (Histidine)14
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Format
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Liquid
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Beschreibung
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Determination of recombinant proteins containing a 5 or 6 amino acid HIS-tag by Western Blotting, ELISA, Flow Cytometry.
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Reinigung
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This antibody was produced by antigen dependent affinity chromatography of positive sera subsequently to immunization of NZW rabbits with Cys–(Histidine)14 coupled to KLH. The purity of antibody fractions was determined by Coomassie staining upon SDS-PAGE. Subsequently the antibody was conjugated to HRP by periodate coupling. Functional analysis of the purified antibody revealed strong and specific reactivity against recombinant HIS-tag proteins by Western Blotting, ELISA and Flow Cytometry. Hereby it makes no difference if the HIS-tag of the target protein is fused to the N- or the C-terminus.
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Buffer
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PBS
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Lagerung
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At least 12 months when stored at –24°C. At least 4 weeks at 4°C when stored properly.
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Forschungsgebiet
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Tags/Labels
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "HIS Tag", Typ "Antikörper" finden
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Wirte
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Maus (82), Kaninchen (58), Huhn (11), Ziege (7), Schaf (1)
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Reaktivitäten
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Fusion Protein (Tag) (14), Human (11), Alle Spezies (10), Maus (8), Ratte (Rattus) (6), Tag (4), Diverse Spezies (2), Rind (Kuh) (1), Hund (1), Schwein (1), Kaninchen (1)
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Applikationen
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Western Blot (WB) (117), ELISA (74), Immunpräzipitation (IP) (50), Immunfluoreszenz (IF) (45), Durchflusszytometrie (FACS) (30), Immunzytochemie (ICC) (13), Immunhistochemie (IHC) (10), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (6), Dot Blot (Dot) (5), Immunhistochemie (Paraffinschnitte) (IHC (p)) (5), Transfizierte Zellkultur (TCC) (3), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), Immunoelectron Microscopy (IEM) (1), Immunostaining (ISt) (1)
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Konjugate
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HRP (11), Biotin (10), FITC (8), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Alkaline Phosphatase (AP) (2), HiLyte Fluor 488 (2), HiLyte Fluor 555 (2), HiLyte Fluor 647 (2), PE,Cy5.5 (2), AMCA (1), Alexa Flour 488 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FAM (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), RBITC (1), ROX (1), Sepharose (1), TAMRA (1)
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