HIS Tag Antikörper

Antigen: HIS Tag

Klonalität: Polyklonal

Wirt: Kaninchen

Applikation: Western Blot (WB), ELISA, Immunpräzipitation (IP), Durchflusszytometrie (FACS), Immunhistochemie (IHC)

Produktnummer: ABIN195462

Produktbeschreibung

Weitere Bezeichnung: HIS-tag

Immunogen: Cys – (Histidine)14

Format: Liquid

Beschreibung: Determination of recombinant proteins containing a 5 or 6 amino acid HIS-tag by Western Blotting, ELISA, Immunoprecipitation , Flow Cytometry, Immunohistochemistry

Anwendungen

Reinigung: This antibody was produced by antigen dependent affinity chromatography of positive sera subsequently to immunization of NZW rabbits with Cys–(Histidine)14 coupled to KLH. The purity of antibody fractions was determined by Coomassie staining upon SDS-PAGE. Functional analysis of the purified antibody revealed strong and specific reactivity against recombinant HIS-tag proteins by Western Blotting, Immunoprecipitation, ELISA, Flow Cytometry and Immunohistochemistry. Hereby it makes no difference if the HIS-tag of the target protein is fused to the N- or the C-terminus.

Buffer: PBS

Lagerung: At least 18 months when stored at –24°C. At least 4 weeks at 4°C when stored properly.

Forschungsgebiet: Tags/Labels

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Determination of a bacterially expressed recombinant 40kD protein with antiHIS-tag by Western Blot. Protein extracts of a negative (A) and a positive bacterial strain (B) were resolved by SDS-PAGE and subsequently transferred to PVDF membrane. The target protein was determined by antiHis-tag (1µg/ml) followed by Anti-Rabbit-HRP and ECL detection. The marks indicated on the left represent the prestained protein standard used in the experiment with individual bands of 23,1 kD, 31,3 kD, 53,6 kD and 100,4 kD respectively from bottom to the top.