Hellethionin Antikörper

Antigen: Hellethionin

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Helleborus purpurascens

Applikation: ELISA, Western Blot (WB)

Produktnummer: ABIN195437

Produktbeschreibung

Immunogen: mixture of extracted hellethionins A, B, C, D, E and G from Helleborus purpurascens conjugated to ovalbumin

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Hellethionins (Milbradt et el. 2003) belong to a family of plant thionins, a small-sized multiple-cysteine peptides that are involved in the plant defence against pathogens. The antimicrobial activity and cytotoxicity of thionins have led to the development of two potential applications of thionins: in agriculture transgenic plants containing thionin genes can enhance pathogen resistance, and targeting of thionins by tumor-specific antibodies is expected to support antitumor therapy.

Spezifität: Reacts with Hellethionin A, B, C, D, E and G from H. purpurascens. The crossreaction with other plant thionins was not analysed.

Anwendungen

Applikationshinweise: ELISA (1 : 256.000), Western blot (1 : 500 for ECL)

Buffer: contains 0.01% NaN3

Lagerung: short term +4°C; long term -20°C. Repeated freezing and thawing is not recommended.

Forschungsgebiet: Pflanzenphysiologie

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western blot analysis extracted hellethionins B, C and D with anti-hellethionin. M– monomer, d – dimer forms of hellethionin.