»
Antikörper
»
anti-HLA-DR-gamma (CD74) Antikörper
HLA-DR-gamma (CD74) Antikörper
| Antigen |
|
| Synonyme |
DHLAG, HLADG, Ia-GAMMA, Ii, CLIP |
| Klonalität |
Monoklonal (LN2) |
|
Wirt
|
|
|
Reaktivität
|
Human (195), Hund (46), Rhesusaffen (45), Pavian (32), Cynomolgus (30), Ratte (Rattus) (21), Maus (20), Schwein (18), Rind (Kuh) (17), Pferd (17), Schaf (15), Schimpanse (6), Marmoset (6), Tamarin (6), Affe (2), Kaninchen (2), Huhn (1)
|
|
Applikation
|
Alternativen Durchflusszytometrie (FACS), Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p))
Durchflusszytometrie (FACS) (160), Immunpräzipitation (IP) (53), Immunhistochemie (Paraffinschnitte) (IHC (p)) (44), Western Blot (WB) (34), Immunfluoreszenz (IF) (28), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (25), Immunhistochemie (IHC) (23), Immunzytochemie (ICC) (5), ELISA (4), Blockierende Antikörper (Inhibition) (2), Enzyme Immunoassay (EIA) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1)
|
| Produktnummer |
ABIN192403 |
| Menge |
0.1 mg (1 mg/ml) (Varianten) |
| Preis |
180,00 € Zzgl. Versandkosten €40,00 und MWSt
|
| Lieferung nach |
|
| Verfügbarkeit |
Lieferung in 3 bis 5 Werktagen |
|
Weitere Bezeichnung
|
CD74
|
|
Immunogen
|
SU-DHL-4 lymphoma cells
|
|
Format
|
Purified
|
|
Isotyp
|
IgG1 (Passende Sekundärantikörper)
|
|
Klon
|
LN2
|
|
Beschreibung
|
CD74 (the MHC II-associated invariant chain, Ii) is an type II transmembrane proteinexpressed in antigen-presenting cells, that serves as MHC II chaperone which promotesMHC II trafficking from the ER to endocytic compartments, prevents peptide binding in theER and contributes to peptide editing in the MHC II compartment, it is also an accessorysignaling molecule implicated e.g. in malignant B cell proliferation. Stimulation of cell surfaceCD74 leads to NFkappaB activation, which enables entry of the stimulated cell into the Sphase. CD74 binds pro-inflammatory cytokine MIF with high affinity and interacts with CD44.Binding of Vpu, an HIV1 protein, to CD74 modulates MHC II presentation.
|
|
Spezifität
|
The antibody LN2 is specific to CD74. It also reacts with activated neoplastic cells in T celllymphomas, lymph node germinal center, mantle zone B cells, histiocytes, interdigitatingreticulum cells, Langerhans cells, thymic dendritic cells and peripheral blood B lymphocytes.HLDA IV, WS Code B 129HLDA V, WS Code B CD74.3
|
|
Applikationshinweise
|
Immunohistochemistry (paraffin sections) - Recommended dilution: 10 µg/ml
|
|
Konzentration
|
1 mg/ml
|
|
Reinheit
|
> 95% (by SDS-PAGE)
|
|
Reinigung
|
Purified from hybridoma culture supernatant by protein-A affinity chromatography.
|
|
Buffer
|
Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
|
|
Lagerung
|
Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
|
|
Forschungsgebiet
|
Immunologie, Adaptive Immunität, CD Antigene, Oberflächenrezeptoren der Immunzellen , Haupthistokompatibilitätskomplex (MHC)
|
|
Beschränkungen
|
Nur für Forschungszwecke einsetzbar
|
Alternativen zu Antigen "HLA-DR-gamma (CD74)", Typ "Antikörper" finden
|
Wirte
|
Maus (164), Kaninchen (25), Ratte (9)
|
|
Reaktivitäten
|
Human (195), Hund (46), Rhesusaffen (45), Pavian (32), Cynomolgus (30), Ratte (Rattus) (21), Maus (20), Schwein (18), Rind (Kuh) (17), Pferd (17), Schaf (15), Schimpanse (6), Marmoset (6), Tamarin (6), Affe (2), Kaninchen (2), Huhn (1)
|
|
Applikationen
|
Durchflusszytometrie (FACS) (160), Immunpräzipitation (IP) (53), Immunhistochemie (Paraffinschnitte) (IHC (p)) (44), Western Blot (WB) (34), Immunfluoreszenz (IF) (28), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (25), Immunhistochemie (IHC) (23), Immunzytochemie (ICC) (5), ELISA (4), Blockierende Antikörper (Inhibition) (2), Enzyme Immunoassay (EIA) (2), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (2), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1)
|
|
Konjugate
|
FITC (22), Biotin (16), PE (16), Alexa Fluor 647 (7), APC (6), Alexa Fluor 488 (6), Alexa Fluor 700 (6), Pacific Blue (6), PE,Cy5 (3), PE,Cy7 (3), PerCP (3), APC,Cy7 (2), PerCP,Cy5.5 (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 555 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5.5 (1), RBITC (1), RPE (1)
|