Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A) Antikörper (PE-Dyomics 647)

Antigen: Fc Fragment of IgG, Low Affinity IIIa, Receptor (CD16a) (FCGR3A)

Synonyme: CD16, FCG3, CD16A, FCGR3, IGFR3, FCR-10, FCRIII, FCGRIII, FCRIIIA

Klonalität: Monoklonal (LNK16)

Wirt: Maus

Reaktivität: Human, Primaten

Konjugat: PE-Dyomics 647

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN192313

Produktbeschreibung

Weitere Bezeichnung: CD16

Immunogen: Normal human peripheral blood granulocytes

Isotyp: IgG1

Klon: LNK16

Beschreibung: CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor.Human FcgammaRIII is expressed in two forms FcgammaRIII-A and -B. FcgammaRIII-A isa transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It isassociated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NKcell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-betasubunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE.FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and isinvolved in their activation and induction of a proadhesive phenotype.

Spezifität: The antibody LNK16 reacts with CD16, a low affinity receptor for aggregated IgG(FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA,50-65 kDa, expressed on NK-cells, monocytes and macrophages) and CD16b(FcgammaRIIIB, 48 kDa, mainly expressed on neutrophils).HLDA V, WS Code M MA069HLDA V, WS Code NK NK50

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Flow Cytometry analysis

Publikationen

Publikationen: Mathison, Befus, Davison et al.: "Modulation of neutrophil function by the tripeptide feG." in: BMC immunology, Vol. 4, pp. 3, 2003 (PubMed).

Lin, Liu, Chen et al.: "CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features." in: Blood, Vol. 106, Issue 10, pp. 3567-74, 2005 (PubMed).