Collagen, Type XVII, alpha 1 (COL17A1) (AA 1-452) Antikörper

Antigen: Collagen, Type XVII, alpha 1 (COL17A1)

Synonyme: BP180, BPAG2, LAD-1, FLJ60881, KIAA0204, BA16H23.2, Bpag, Bpag2, COL17A1, MGC155048, fk89a06, wu:fk89a06, si:ch211-223p8.6

Epitope: AA 1-452

Klonalität: Monoklonal (V-5-8)

Wirt: Maus

Reaktivität: Human

Applikation: Immunhistochemie (Gefrierschnitte) (IHC (fro)), Enzyme Immunoassay (EIA), Western Blot (WB)

Produktnummer: ABIN192031

Produktbeschreibung

Produktmerkmale: Synonyms: COL17A1, BP180, BPAG2, Bullous pemphigoid antigen 2, LAD-1, LABD97, 180 kDa bullouspemphigoid antigen 2

Weitere Bezeichnung: Collagen type XVII alpha 1 chain

Gen-ID: 1308

Swiss-Prot: Q9UMD9

Immunogen: Human type XVII collagen intracellular domain (aa 1-490) GST fusion protein

Kreuzreaktivität: Human

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: V-5-8

Beschreibung: Unlike most collagens, collagen XVII is a transmembrane protein. Collagen XVII is astructural component of hemidesmosomes, multiprotein complexes at the dermalepidermal basement membrane zone that mediate adhesion of keratinocytes to theunderlying membrane. Mutations in the gene coding for collagen XVII are associated withboth generalized atrophic benign and junctional epidermolysis bullosa. Two homotrimericforms of type XVII collagen exist. The full length form is the transmembrane protein. Asoluble form, referred to as either ectodomain or LAD 1, is generated by proteolyticprocessing of the full length form. Two transcript variants, one resulting from alternativesplicing in the 3' UTR, have been identified for this gene.

Spezifität: This antibody detects Human type XVII Collagen (234-398), Native Human type XVIICollagen. Species: Human. Others not tested.

Anwendungen

Applikationshinweise: ELISA: 1/1000. Western blot: 1/100. Immunohistpochemitry on Frozen Sections: 1/100. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Reinigung: Purified

Buffer: 50 mM TRIS pH 7.4Reconstitution: Restore in aqua bidest to 1.0 mg/ml.

Lagerung: Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Figure 1. V-5-8 mAb labels the dermal-epidermal junction by immunofluorescence microscopy. A 1:100 dilution of purified V-5-8 IgG was incubated 30 min at room temprature with frozen sections of human skin. After washing, bound IgG was visualized using FITC-conjugated antibodies specific for mouse (B) IgG and (C) IgG2a (all magnification, x400).